Su Xiaoqin, Cui Yanqiu, Gong Hanfu, Xu Tianjun, Sun Yuena
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China; Marine Biomedical Science and Technology Innovation Platform of Lin-gang Special Area, Shanghai, China.
Fish Shellfish Immunol. 2025 Aug;163:110369. doi: 10.1016/j.fsi.2025.110369. Epub 2025 Apr 25.
The tumor necrosis factor superfamily (TNFSF) is crucial in regulating immune responses, with its members mediating various biological functions through key signaling pathways. However, the gene characteristics of this family and their comparative and evolutionary analysis across species remain limited. In this study, 12 TNFSF genes were identified in the genome-wide of miiuy croaker. Analyses were conducted on evolutionary relationships, conserved motifs, gene duplication, and selection pressure. Conserved motif analyses revealed that the C-terminal motifs of vertebrate TNFSF proteins were more conserved than the N-terminus. Sequence alignment and conservation analysis identified an unrecognized helix structure within the TNF homology domain, which exhibited structural conservation among vertebrates. Synteny and selection pressure analyses indicated that the TNFSF in miiuy croaker exhibited tandem and segmental duplication events. Evolutionary selection pressures may contributed to the functional differentiation of this family. These findings could enhance the understanding of TNFSF gene characteristics and evolutionary relationships, and provide new insights for studying immune-related TNFSF genes.
肿瘤坏死因子超家族（TNFSF）在调节免疫反应中至关重要，其成员通过关键信号通路介导多种生物学功能。然而，该家族的基因特征及其在不同物种间的比较和进化分析仍然有限。在本研究中，在黄斑海鲈全基因组中鉴定出12个TNFSF基因。对其进化关系、保守基序、基因复制和选择压力进行了分析。保守基序分析表明，脊椎动物TNFSF蛋白的C端基序比N端更保守。序列比对和保守性分析在TNF同源结构域内鉴定出一种未被识别的螺旋结构，该结构在脊椎动物中表现出结构保守性。共线性和选择压力分析表明，黄斑海鲈中的TNFSF表现出串联和片段重复事件。进化选择压力可能促成了该家族的功能分化。这些发现有助于增进对TNFSF基因特征和进化关系的理解，并为研究免疫相关的TNFSF基因提供新的见解。
