Ruiz Daniels R, Salisbury S J, Sveen L, Villamayor P R, Taylor R S, Vaadal M, Tengs T, Krasnov A, Monaghan S J, Ballantyne M, Penaloza C, Fast M D, Bron J E, Houston R, Robinson N, Robledo D
The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK.
Institute of Aquaculture, University of Stirling, Stirling, UK.
BMC Biol. 2025 Apr 28;23(1):109. doi: 10.1186/s12915-025-02196-w.
The skin maintains the body's integrity and serves as the first line of defence against pathogens, stressors and mechanical injuries. Despite the global significance of salmon in aquaculture, how the transcriptomic profile of cells varies during wound healing remains unexplored. Teleost's skin contains adult pluripotent cells that differentiate into various tissues, including bone, cartilage, tendon, ligament, adipose, dermis, muscle and connective tissue within the skin. These cells are pivotal for preserving the integrity of skin tissue throughout an organism's lifespan and actively participate in the wound healing processes. In this study, we characterize the transcriptomic profiles of putative mesenchymal stromal cells (fibroblast-like adult stem cells) in healthy Atlantic salmon tissue and during the wound healing process.
Single-nucleus sequencing and spatial transcriptomics were used to detect transcriptomic changes occurring during wound healing that are commonly associated with mesenchymal stromal cells. We followed the transcriptomic activity of these cells during an in vivo wound healing time course study showing that these cells become more transcriptionally active during the remodelling stage of wound healing. The changes detected give insights into the potential differentiation pathways leading to osteogenic and fibroblast lineages in the skin of Atlantic salmon.
We chart the transcriptomic activity of subclusters of putative differentiating stromal cells during the process of wound healing for the first time, revealing different spatial niches of the various putative MSC subclusters, and setting the stage for further investigation of the manipulation of transitioning cell types to improve fish health.
皮肤维持身体的完整性，是抵御病原体、应激源和机械损伤的第一道防线。尽管鲑鱼在水产养殖中具有全球重要性，但细胞转录组图谱在伤口愈合过程中如何变化仍未得到探索。硬骨鱼的皮肤含有成体多能细胞，这些细胞可分化为各种组织，包括骨骼、软骨、肌腱、韧带、脂肪、真皮、肌肉和皮肤内的结缔组织。这些细胞对于在生物体的整个生命周期中保持皮肤组织的完整性至关重要，并积极参与伤口愈合过程。在本研究中，我们对健康大西洋鲑鱼组织和伤口愈合过程中假定的间充质基质细胞（成纤维细胞样成体干细胞）的转录组图谱进行了表征。
使用单核测序和空间转录组学来检测伤口愈合过程中发生的、通常与间充质基质细胞相关的转录组变化。在一项体内伤口愈合时间进程研究中，我们追踪了这些细胞的转录组活性，结果表明这些细胞在伤口愈合的重塑阶段转录活性更高。检测到该变化为深入了解导致大西洋鲑鱼皮肤中骨生成和成纤维细胞谱系的潜在分化途径提供了线索。
我们首次绘制了假定的分化基质细胞亚群在伤口愈合过程中的转录组活性，揭示了各种假定的间充质干细胞亚群的不同空间生态位，并为进一步研究操纵过渡细胞类型以改善鱼类健康奠定了基础。
