Garattini E, Gazzotti G, Salmona M
Experientia. 1981 Mar 15;37(3):230-1. doi: 10.1007/BF01991626.
The apparent Km and Vmax of styrene monooxygenase and styrene epoxide hydrolase were determined in intact nuclear preparations from male rat liver after in vivo treatment with phenobarbital and beta-naphthoflavone, which are known to induce microsomal cytochrome P-450 and cytochrome P-448 respectively. Treatment with phenobarbital does not alter the apparent Km, but greatly increases the Vmax of both nuclear styrene monooxygenase and styrene epoxide hydrolase. Almost the same pattern is observed for styrene monooxygenase after treatment with beta-naphthoflavone, whereas the same treatment slightly increases both the Vmax and Km value of styrene epoxide hydrolase.
在用苯巴比妥和β-萘黄酮进行体内处理后，从雄性大鼠肝脏的完整细胞核制剂中测定了苯乙烯单加氧酶和苯乙烯环氧化物水解酶的表观米氏常数（Km）和最大反应速度（Vmax）。已知苯巴比妥和β-萘黄酮分别诱导微粒体细胞色素P-450和细胞色素P-448。用苯巴比妥处理不会改变表观Km，但会大大增加细胞核苯乙烯单加氧酶和苯乙烯环氧化物水解酶的Vmax。用β-萘黄酮处理后，苯乙烯单加氧酶观察到几乎相同的模式，而相同处理会使苯乙烯环氧化物水解酶的Vmax和Km值略有增加。
