Wang Jenny S H, Rodolf Amelia A, Moon Caleb H, Lauthner Ari, Vu Helen H, Rugonyi Sandra, Hansen Anna J, Mayes Heather M, Zakhary Bishoy, Zonies David, Ran Ran, Khan Akram, Wirtz Denis, Kiemen Ashley L, McCarty Owen J T, Shatzel Joseph J
Department of Biomedical Engineering, Oregon Health & Science University, 3303 S Bond Ave, Portland, OR 97239 USA.
Division of Nursing, Cardiovascular Intensive Care, Oregon Health & Science University, Portland, OR USA.
Cell Mol Bioeng. 2025 Apr 12;18(2):197-209. doi: 10.1007/s12195-025-00847-0. eCollection 2025 Apr.
Extracorporeal membrane oxygenation (ECMO) is a life-saving critical care technology that presents significant risks of medical device-associated thrombosis. We developed a complete method for collecting membrane oxygenators (membrane lung) from patients receiving ECMO treatment and quantitatively analyzing the distribution of thrombus formation within the membrane.
We collected used membrane oxygenators from patients for processing and imaging with microcomputed tomography (microCT). We reconstructed the microCT data and performed image segmentation to identify regions of thrombus formation within these oxygenators. We performed density mapping to quantify thrombus volume across different regions of each oxygenator and within multiple oxygenator models.
Our method yields two-dimensional and three-dimensional visualization and quantification of thrombus deposition in ECMO. Analysis of the spatial distribution of platelet deposition, red blood cell entrapment, and fibrin formation within the fouled device provides insights into the structural patterns of oxygenator thrombosis.
This method can enable quantification of oxygenator thrombosis which can be used for evaluating the effect of new biomaterial or pharmacological approaches for mitigating vascular device-associated thrombosis during ECMO.
The online version contains supplementary material available at 10.1007/s12195-025-00847-0.
体外膜肺氧合（ECMO）是一种挽救生命的重症监护技术，但存在与医疗器械相关的血栓形成的重大风险。我们开发了一种完整的方法，用于从接受ECMO治疗的患者身上收集膜式氧合器（膜肺），并定量分析膜内血栓形成的分布情况。
我们从患者身上收集用过的膜式氧合器，以便用微型计算机断层扫描（microCT）进行处理和成像。我们重建了microCT数据，并进行图像分割，以识别这些氧合器内血栓形成的区域。我们进行密度映射，以量化每个氧合器不同区域以及多个氧合器模型内的血栓体积。
我们的方法可实现对ECMO中血栓沉积的二维和三维可视化及量化。对污染装置内血小板沉积、红细胞截留和纤维蛋白形成的空间分布分析，有助于深入了解氧合器血栓形成的结构模式。
该方法能够对氧合器血栓形成进行量化，可用于评估新型生物材料或药理学方法在减轻ECMO期间血管装置相关血栓形成方面的效果。
在线版本包含可在10.1007/s12195-025-00847-0获取的补充材料。
