Wang Zhifen, Giedraitis Emily, Knoop Christiane, Breiner Daniel J, Phelan Vanessa V, Van Bambeke Françoise
Pharmacologie Cellulaire et moléculaire, Louvain Drug Research Institute, Université Catholique de Louvain, Brussels, Belgium.
Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Biofilm. 2025 Apr 11;9:100279. doi: 10.1016/j.bioflm.2025.100279. eCollection 2025 Jun.
Co-infections by and are frequent in the airways of patients with cystic fibrosis. These co-infections show higher antibiotic tolerance compared to mono-infections. models have been developed to study the interspecies interactions between and . However, these model systems fail to incorporate clinical isolates with diverse phenotypes, do not reflect the nutritional environment of the CF airway mucus, and/or do not model the biofilm mode of growth observed in the CF airways. Here, we established a dual-species biofilm model grown in artificial sputum medium, where was inoculated before to facilitate the maintenance of both species over time. It was successfully applied to ten pairs of clinical isolates exhibiting different phenotypes. Co-isolates from individual patients led to robust, stable co-cultures, supporting the theory of cross-adaptation . Investigation into the cross-adaptation of the VBB496 co-isolate pair revealed that both the and isolates had reduced antagonism, in part due to reduced production of secondary metabolites as well as higher tolerance to those metabolites by . Together, these results indicate that the two-species biofilm model system provides a useful tool for exploring interspecies interactions of and in the context of CF airway infections.
在囊性纤维化患者的气道中，[具体细菌1]和[具体细菌2]的合并感染很常见。与单一感染相比，这些合并感染表现出更高的抗生素耐受性。已经开发了[具体模型名称]来研究[具体细菌1]和[具体细菌2]之间的种间相互作用。然而，这些模型系统未能纳入具有不同表型的临床分离株，没有反映囊性纤维化气道黏液的营养环境，和/或没有模拟在囊性纤维化气道中观察到的生物膜生长模式。在这里，我们建立了一种在人工痰液培养基中生长的双物种生物膜模型，其中在接种[具体细菌2]之前先接种[具体细菌1]，以便随着时间的推移维持两种细菌的生长。该模型已成功应用于十对表现出不同表型的临床分离株。来自个体患者的共分离株产生了强大、稳定的共培养物，支持了交叉适应理论。对VBB496共分离株对的交叉适应研究表明，[具体细菌1]和[具体细菌2]分离株的拮抗作用均有所降低，部分原因是[具体细菌1]次生代谢产物的产生减少以及[具体细菌2]对这些代谢产物的耐受性提高。总之，这些结果表明，双物种生物膜模型系统为探索囊性纤维化气道感染背景下[具体细菌1]和[具体细菌2]的种间相互作用提供了一个有用的工具。
