Srikant M, Sandra Nagamani, Kumar Atul, Lal Sandeep Kumar, Lal Sanjay Kumar, Mandal Bikash
Seed Pathology Laboratory, Division of Seed Science and Technology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.
Virusdisease. 2025 Mar;36(1):41-47. doi: 10.1007/s13337-024-00905-7. Epub 2025 Jan 23.
is an emerging viral pathogen in leguminous crops including soybean. The present study was carried with 18 soybean cultivars to screen and identify the resistant cultivars to SYMMV infection under controlled environment glasshouse. Agro-inoculation of French bean cv. Arka Sharat with SYMMV produced systemic symptoms of mosaic and chlorotic blotches. Mechanical sap inoculation of 18 soybean cultivars with SYMMV infected French bean leaves produced systemic symptoms like chlorotic spots, chlorotic blotches, mosaic, mottle and veinal mild mottling by 15-20 dpi. The PDI on a scale of 0-5 showed that out of 18 cultivars, only SL-979 cultivar was moderately resistant, seven cultivars were moderately susceptible, and eight cultivars were susceptible, while two cultivars displayed highly susceptible reaction (SL-958 & JS-335). Detection of SYMMV through DAC-ELISA showed the absorbance values of 0.84 to 2.05 at 405 nm. RT-PCR analysis of these cultivars showed amplification of 1065 bp fragment with CP primers. The present study clearly showed that none of the screened soybean cultivar is resistant against SYMMV, suggesting a need for further research into breeding programs that may enhance resistance to SYMMV.
The online version contains supplementary material available at 10.1007/s13337-024-00905-7.
是包括大豆在内的豆科作物中一种新出现的病毒病原体。本研究使用18个大豆品种，在可控环境温室中筛选和鉴定对大豆褪绿斑驳病毒（SYMMV）感染具有抗性的品种。用SYMMV对法国菜豆品种Arka Sharat进行农业接种，产生了花叶和褪绿斑点的系统症状。用感染SYMMV的法国菜豆叶片对18个大豆品种进行机械汁液接种，在接种后15 - 20天产生了如褪绿斑点、褪绿斑块、花叶、斑驳和叶脉轻度斑驳等系统症状。以0 - 5级的病情指数（PDI）表示，18个品种中，只有SL - 979品种为中度抗性，7个品种为中度敏感，8个品种为敏感，而2个品种表现出高度敏感反应（SL - 958和JS - 335）。通过双抗夹心酶联免疫吸附测定（DAC - ELISA）检测SYMMV，在405nm处吸光度值为0.84至2.05。对这些品种进行逆转录聚合酶链反应（RT - PCR）分析，用外壳蛋白（CP）引物扩增出1065bp的片段。本研究清楚地表明，所筛选的大豆品种中没有一个对SYMMV具有抗性，这表明需要进一步研究育种计划，以增强对SYMMV的抗性。
在线版本包含可在10.1007/s13337 - 024 - 00905 - 7获取的补充材料。
