Du Ruochen, Guo Ying, Zhong Wanting, Gao Yuantao, Xu Miaomiao, Wang Chunfang, Yuan Yitong
Department of Laboratory Animal Center, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
College of Basic Medical, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Biochem Biophys Rep. 2025 Apr 18;42:102014. doi: 10.1016/j.bbrep.2025.102014. eCollection 2025 Jun.
Gastrodin, a bioactive ingredient extracted from the Chinese herb Gastrodia gastrodia, has shown potential therapeutic effects in cardiovascular diseases, but its specific role in myocardial infarction is unclear. This study investigated the protective effect of gastrodin on myocardial infarction and its potential mechanism. By clamping the left coronary artery, we created a model of myocardial infarction in C57BL/6J mice. For 14 days, mice in the control and myocardial infarction groups received a daily dose of 100 mg/kg gastrodin. Gastrodin significantly improved cardiac dysfunction in mice with myocardial infarction, decreased heart weight/body weight (HW/BW) and heart weight/tibial length (HW/TL) ratios, and inhibited mRNA expression levels of cardiac fibrosis biomarkers (Collagen Type I (Col1), Collagen Type III (Col3), Matrix Metalloproteinase-2 (MMP-2), Matrix Metalloproteinase-9 (MMP-9)). In addition, gastrodin also inhibited the activity of apoptosis marker Caspase 3, decreased the Bax/Bcl2 mRNA ratio, decreased the expression of pro-inflammatory factors (Interleukin-1 beta (IL-1β), Tumor Necrosis Factor-alpha (TNF-α), Interleukin-6 (IL-6)) and pro-inflammatory adhesion molecule Monocyte Chemoattractant Protein-1 (MCP1), and promoted the expression of angiogenesis marker Cluster of Differentiation 31 (CD31). RNA sequencing and Rt-qPCR analysis showed that gastrodin treatment significantly up-regulated the expression of genes related to cell proliferation (Cyclin-Dependent Kinase 1 (CDK1), Threonine Tyrosine Kinase (TTK), Cyclin B2 (CCNB2), Polo-Like Kinase 1 (PLK1)), and promoted the proliferation of human aortic endothelial cells (HAECs). These findings suggest that gastrodin can effectively reduce the pathological changes of myocardial infarction by inhibiting inflammation, reducing apoptosis, and promoting endothelial cell proliferation, thus providing a new strategy for the prevention and treatment of myocardial infarction.
天麻素是从中药材天麻中提取的一种生物活性成分，已显示出在心血管疾病中的潜在治疗作用，但其在心肌梗死中的具体作用尚不清楚。本研究探讨了天麻素对心肌梗死的保护作用及其潜在机制。通过夹闭左冠状动脉，我们在C57BL/6J小鼠中建立了心肌梗死模型。连续14天，对照组和心肌梗死组小鼠每日给予100mg/kg天麻素。天麻素显著改善了心肌梗死小鼠的心功能障碍，降低了心脏重量/体重（HW/BW）和心脏重量/胫骨长度（HW/TL）比值，并抑制了心脏纤维化生物标志物（I型胶原（Col1）、III型胶原（Col3）、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9））的mRNA表达水平。此外，天麻素还抑制了凋亡标志物半胱天冬酶3的活性，降低了Bax/Bcl2 mRNA比值，降低了促炎因子（白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6））和促炎黏附分子单核细胞趋化蛋白-1（MCP1）的表达，并促进了血管生成标志物分化簇31（CD31）的表达。RNA测序和Rt-qPCR分析表明，天麻素处理显著上调了细胞增殖相关基因（细胞周期蛋白依赖性激酶1（CDK1）、苏氨酸酪氨酸激酶（TTK）、细胞周期蛋白B2（CCNB2）、波罗蛋白样激酶1（PLK1））的表达，并促进了人主动脉内皮细胞（HAECs）的增殖。这些发现表明，天麻素可通过抑制炎症、减少凋亡和促进内皮细胞增殖有效减轻心肌梗死的病理变化，从而为心肌梗死的防治提供新策略。
