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以热响应水凝胶为培养支架的微流控芯片上的SKOV-3细胞聚集体用于阿霉素评估

SKOV-3 Cell Aggregates on a Microfluidic Chip with a Thermoresponsive Hydrogel as a Culture Scaffold for DOX Assessment.

作者信息

Zhang Tianzhu, Yang Liuxin, Wang Zhengyang, Zhou Naizhen

机构信息

State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

出版信息

ACS Omega. 2025 Apr 9;10(15):14972-14979. doi: 10.1021/acsomega.4c10301. eCollection 2025 Apr 22.

DOI:10.1021/acsomega.4c10301
PMID:40290958
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12019744/
Abstract

Microfluidic chip technology is very popular in life sciences. Here, ovarian cancer SKOV-3 cell aggregates were formed using thermoresponsive poly(-isopropylacrylamide--acrylic acid) (PNA) hydrogel as a culture scaffold on a microfluidic chip serving as an operating platform. A simple microfluidic chip was designed and fabricated as the three-dimensional (3D) cell culture microplatform. Different concentrations of doxorubicin (DOX) were fed to the obtained SKOV-3 cell aggregates on the chip via a pump. All characterization results indicated that this system could effectively perform 3D cell culture and drug evaluation to a certain extent. In addition, by grafting the RGD sequence, the biocompatibility of the PNA hydrogel was improved. On the one hand, the grafting of the RGD sequence into the hydrogel could significantly improve cell proliferation in this system; on the other hand, it led to an earlier appearance of DOX drug resistance. This versatile model in this study has the potential for further use in in vitro human ovarian cancer physiological models, drug discovery, and toxicology research.

摘要

微流控芯片技术在生命科学领域非常受欢迎。在此，以热响应性聚（-异丙基丙烯酰胺-丙烯酸）（PNA）水凝胶作为培养支架，在作为操作平台的微流控芯片上形成了卵巢癌SKOV-3细胞聚集体。设计并制造了一种简单的微流控芯片作为三维（3D）细胞培养微平台。通过泵将不同浓度的阿霉素（DOX）输送到芯片上获得的SKOV-3细胞聚集体中。所有表征结果表明，该系统在一定程度上能够有效地进行3D细胞培养和药物评估。此外，通过接枝RGD序列，提高了PNA水凝胶的生物相容性。一方面，将RGD序列接枝到水凝胶中可显著提高该系统中的细胞增殖；另一方面，它导致DOX耐药性更早出现。本研究中的这种通用模型有潜力进一步用于体外人类卵巢癌生理模型、药物发现和毒理学研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/062b/12019744/d12619a1a48e/ao4c10301_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/062b/12019744/5416e01fb266/ao4c10301_0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/062b/12019744/d12619a1a48e/ao4c10301_0009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/062b/12019744/750dde027751/ao4c10301_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/062b/12019744/58344b9d2de9/ao4c10301_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/062b/12019744/3d9f91a1bb80/ao4c10301_0007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/062b/12019744/d12619a1a48e/ao4c10301_0009.jpg

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本文引用的文献

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2
Global Literature Analysis of Organoid and Organ-on-Chip Research.类器官和器官芯片研究的全球文献分析。
Adv Healthc Mater. 2024 Aug;13(21):e2301067. doi: 10.1002/adhm.202301067. Epub 2023 Aug 7.
3
Recent advances in mechanical force-responsive drug delivery systems.机械力响应药物递送系统的最新进展。
Nanoscale Adv. 2022 Jul 18;4(17):3462-3478. doi: 10.1039/d2na00420h. eCollection 2022 Aug 23.
4
Effect of Chitosan on Alginate-Based Macroporous Hydrogels for the Capture of Glioblastoma Cancer Cells.壳聚糖对用于捕获胶质母细胞瘤癌细胞的海藻酸盐基大孔水凝胶的影响。
ACS Appl Bio Mater. 2022 Aug 10. doi: 10.1021/acsabm.2c00598.
5
microenvironment remodeling using a dual-responsive system: photodegradable hydrogels and gene activation by visible light.使用双响应系统进行微环境重塑：可光降解水凝胶与可见光介导的基因激活
Biomater Sci. 2022 Jul 12;10(14):3981-3992. doi: 10.1039/d2bm00617k.
6
Encapsulation of Cells in a Collagen Matrix Surrounded by an Alginate Hydrogel Shell for 3D Cell Culture.在海藻酸钙水凝胶壳的包围下，将细胞包封在胶原基质中用于 3D 细胞培养。
ACS Biomater Sci Eng. 2022 Jun 13;8(6):2700-2708. doi: 10.1021/acsbiomaterials.1c01486. Epub 2022 May 24.
7
Actuated 3D microgels for single cell mechanobiology.用于单细胞机械生物学的驱动 3D 微凝胶。
Lab Chip. 2022 May 17;22(10):1962-1970. doi: 10.1039/d2lc00203e.
8
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Cells Tissues Organs. 2022;211(3):294-303. doi: 10.1159/000514580. Epub 2021 May 26.
9
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ACS Biomater Sci Eng. 2020 Jun 8;6(6):3310-3326. doi: 10.1021/acsbiomaterials.9b01967. Epub 2020 Apr 21.
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