Sonderegger P, Lemkin P F, Lipkin L E, Nelson P G
EMBO J. 1985 Jun;4(6):1395-401. doi: 10.1002/j.1460-2075.1985.tb03792.x.
Axonal behavior during the formation of the neuronal network of the nervous system has been shown to be under environmental control. Hence, as a first step in a project aiming to elucidate the molecular basis of axonal functions, we have identified axonal proteins whose synthesis is subject to environmentally induced changes. Neurons from chicken embryonic dorsal root ganglia (DRG) were grown in a compartmental cell culture system that allows selective examination of axonal proteins. Non-neuronal cells of the peripheral or central nervous system were co-cultured with the DRG axons. The axonal proteins expressed under these different environmental conditions were examined by metabolic labeling and two-dimensional SDS-polyacrylamide gel electrophoresis. Computerized quantification revealed that 12 out of 400 axonal proteins responded to changes in the local axonal environment by a change in their relative abundance. Some proteins changed in response to both types of co-cultures whereas some changed specifically under the influence of either peripheral or central non-neuronal cells.
神经系统神经网络形成过程中的轴突行为已被证明受环境控制。因此，作为旨在阐明轴突功能分子基础的项目的第一步，我们鉴定了其合成受环境诱导变化影响的轴突蛋白。鸡胚背根神经节（DRG）的神经元在一个分隔细胞培养系统中生长，该系统允许对轴突蛋白进行选择性检测。外周或中枢神经系统的非神经元细胞与DRG轴突共培养。通过代谢标记和二维SDS-聚丙烯酰胺凝胶电泳检测在这些不同环境条件下表达的轴突蛋白。计算机定量分析显示，400种轴突蛋白中有12种因其相对丰度的变化而对局部轴突环境的变化作出反应。一些蛋白质对两种共培养类型均有反应，而一些蛋白质则在外周或中枢非神经元细胞的影响下发生特异性变化。
