Conia J, Edwards B S, Voelkel S
Cell Robotics Inc., Albuquerque, NM 87107, USA.
J Clin Lab Anal. 1997;11(1):28-38. doi: 10.1002/(sici)1098-2825(1997)11:1<28::aid-jcla6>3.0.co;2-g.
With the addition of tightly focused laser beams, microscopes have been turned into elaborate preparative tools that permit not only allow detailed observation of a specimen but also the capture, displacement, and microdissection of biological samples in vitro with astonishing ease and accuracy. Laser-Tweezers are used to capture and manipulate cells and organelles. LaserScissors are used to perform microdissections at the submicrometer level. After a short technical description of the instrumentation and its principles of operation, several examples of applications are given relevant to the field of clinical research that could only be achieved using such modern technology. For instance, LaserTweezers and LaserScissors offer a unprecedented means to study the immune response to cancer, to control the growth of nerve cells, or expand the significance of assisted reproductive technologies. It is suggested that newly developing procedures and assays using laser-assisted technologies will prove beneficial for future clinical laboratory testing.
通过添加高度聚焦的激光束，显微镜已变成精密的制备工具，不仅能够对标本进行详细观察，还能在体外极其轻松且精确地捕获、移动和显微切割生物样本。激光镊子用于捕获和操纵细胞及细胞器。激光剪刀用于在亚微米水平进行显微切割。在对该仪器及其操作原理进行简短的技术描述之后，给出了几个仅使用这种现代技术才能实现的与临床研究领域相关的应用实例。例如，激光镊子和激光剪刀为研究对癌症的免疫反应、控制神经细胞生长或拓展辅助生殖技术的意义提供了前所未有的手段。有人认为，使用激光辅助技术新开发的程序和检测方法将证明对未来的临床实验室检测有益。
