Wasenius V M, Saraste M, Knowles J, Virtanen I, Lehto V P
EMBO J. 1985 Jun;4(6):1425-30. doi: 10.1002/j.1460-2075.1985.tb03797.x.
Immunological screening of a chicken gizzard cDNA expression library was used to isolate two clones encoding a part of the non-erythroid spectrin-like protein. Clones were identified by immunoblotting of the polypeptides synthesized in Escherichia coli cells transformed with cDNA cloned in the pUC8 plasmid vector using polyclonal rabbit antibodies raised against bovine non-erythroid spectrin. The sequence of an approximately 1.5-kb cDNA insert of one clone was determined. Analysis of the predicted amino acid sequence reveals that, despite differences in immunological cross-reactivity and peptide maps, the chicken non-erythroid and the human erythrocyte spectrins are highly homologous proteins. Like the human erythrocyte spectrin, the chicken smooth muscle spectrin appears also to be constructed from repeated, homologous structures of 106 amino acid residues. This is probably a universal structure motif of spectrins.
利用鸡砂囊cDNA表达文库进行免疫筛选，分离出两个编码非红细胞血影蛋白样蛋白部分片段的克隆。通过用针对牛非红细胞血影蛋白产生的多克隆兔抗体，对用克隆于pUC8质粒载体的cDNA转化的大肠杆菌细胞中合成的多肽进行免疫印迹，鉴定出这些克隆。测定了其中一个克隆约1.5kb cDNA插入片段的序列。对预测氨基酸序列的分析表明，尽管在免疫交叉反应性和肽图上存在差异，但鸡非红细胞血影蛋白和人红细胞血影蛋白是高度同源的蛋白质。与人红细胞血影蛋白一样，鸡平滑肌血影蛋白似乎也是由106个氨基酸残基的重复同源结构构成。这可能是血影蛋白的一个普遍结构基序。
