过敏性鼻炎患者血清miR-19b与SOCS-1 mRNA表达水平及临床症状的相关性

The Correlation Between the Expression Levels of Serum miR-19b and SOCS-1 mRNA and Clinical Symptoms in Patients with Allergic Rhinitis.

作者信息

Kang Zhaopeng, Di Lingling, Liu Yi, Qu Wei, Zeng Yi, Lu Xue, Wang Lixin

机构信息

Department of Andrology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, People's Republic of China.

Department of Otolaryngology Head and Neck Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 756099, People's Republic of China.

出版信息

J Asthma Allergy. 2025 Apr 22;18:619-627. doi: 10.2147/JAA.S518177. eCollection 2025.

DOI:10.2147/JAA.S518177

PMID:40292344

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12032965/

Abstract

OBJECTIVE

To investigate the correlation between the expression levels of microRNA-19b (miR-19b) and suppressor of cytokine signaling 1 messenger RNA (SOCS-1 mRNA) in the serum of patients with allergic rhinitis (AR) and clinical symptoms.

METHODS

This prospective study included a total of 86 patients with allergic rhinitis who were admitted to the People's Hospital Affiliated to Hubei University of Medicine from January 2022 to January 2023. Seventy healthy individuals were included in the control group. The case group was further divided into a mild group (n=45) and a moderate to severe group (n=41) according to the severity of AR. The expression levels of miR-19b and SOCS-1 mRNA in serum samples of the two groups were detected and compared using real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) technique. The receiver operating characteristic (ROC) curve and the area under the curve (AUC) were utilized to evaluate the predictive value of miR-19b and SOCS-1 levels for the severity of AR.

RESULTS

The expression level of miR-19b in the serum of the case group was 1.52±0.36, significantly higher than that of the control group (1.02±0.24, P<0.05). Logistic regression analysis indicated that low levels of serum miR-19b and SOCS-1 mRNA were independent risk factors for moderate to severe AR, with an odds ratio (OR) of 3.575 (95% CI: 1.572-8.133, P<0.001) for miR-19b and 3.725 (95% CI: 1.637-8.473, P<0.001) for SOCS-1 mRNA. ROC curve analysis demonstrated that the levels of serum miR-19b and SOCS-1 mRNA had high accuracy in predicting moderate to severe AR, with AUC values of 0.879 (95% CI: 0.791-0.940) and 0.795 (95% CI: 0.694-0.875), respectively. When combined for prediction, the efficacy was significantly higher than that of individual detection, with an AUC value of 0.923 (95% CI: 0.856-0.967), Z=3.261, P<0.01.

CONCLUSION

The expression levels of serum miR-19b and SOCS-1 mRNA are closely related to the severity of clinical symptoms in patients with AR and may serve as new biomarkers for evaluating the condition of AR and guiding treatment.

摘要

目的

探讨变应性鼻炎（AR）患者血清中微小RNA-19b（miR-19b）表达水平与细胞因子信号转导抑制因子1信使核糖核酸（SOCS-1 mRNA）水平及临床症状之间的相关性。

方法

本前瞻性研究共纳入2022年1月至2023年1月在湖北医药学院附属人民医院就诊的86例变应性鼻炎患者。对照组纳入70名健康个体。病例组根据AR严重程度进一步分为轻度组（n=45）和中重度组（n=41）。采用实时荧光定量聚合酶链反应（qRT-PCR）技术检测并比较两组血清样本中miR-19b和SOCS-1 mRNA的表达水平。利用受试者工作特征（ROC）曲线及曲线下面积（AUC）评估miR-19b和SOCS-1水平对AR严重程度的预测价值。

结果

病例组血清中miR-19b表达水平为1.52±0.36，显著高于对照组（1.02±0.24，P<0.05）。Logistic回归分析表明，血清miR-19b和SOCS-1 mRNA低水平是中重度AR的独立危险因素，miR-19b的比值比（OR）为3.575（95%CI：1.572-8.133，P<0.001），SOCS-1 mRNA的OR为3.725（95%CI：1.637-8.473，P<0.001）。ROC曲线分析显示，血清miR-19b和SOCS-1 mRNA水平对中重度AR具有较高预测准确性，AUC值分别为0.879（95%CI：0.791-0.940）和0.795（95%CI：0.694-0.875）。联合预测时，效能显著高于单项检测，AUC值为0.923（95%CI：0.856-0.967），Z=3.261，P<0.01。