AbdElneam Ahmed Ibrahim, Al-Dhubaibi Mohammed Saleh, Bahaj Saleh Salem, Mohammed Ghada Farouk, Atef Lina Mohammed
Department of Clinical Biochemistry, Department of Basic Medical Sciences, College of Medicine, Shaqra University, Dawadmi, Saudi Arabia.
Molecular Genetics and Enzymology Department, National Research Center, Human Genetics and Genome Research Institute, 33 El Bohouth St. (Former El Tahrir St.), Dokki 12622, Cairo, Egypt.
Arch Dermatol Res. 2025 Jan 18;317(1):284. doi: 10.1007/s00403-024-03788-2.
Alopecia areata (AA) is an autoimmune condition marked by hair loss, linked to inflammatory processes involving the interleukin-1 receptor type 1 (IL-1R1) pathway. This study aims to explore the relationship between IL-1R1 gene expression, serum IL-1R1 levels, and hsa-miR-19b-3p in relation to AA severity. Using a case-control design, we assessed 100 AA patients and 100 healthy controls, measuring serum IL-1R1 through enzyme-linked immunosorbent assay (ELISA) and analyzing IL-1R1 gene and hsa-miR-19b-3p expression levels via quantitative real-time PCR (qRT-PCR). Bioinformatic analysis predicted a binding site for hsa-miR-19b-3p on the IL-1R1 gene, suggesting a regulatory role for this miRNA in AA pathology. Demonstrated significantly higher serum IL-1R1, IL-1R1 gene expression, and hsa-miR-19b-3p levels in AA patients compared to controls. Within the AA cohort, severe cases showed the highest levels, with notable correlations between serum IL-1R1, IL-1R1 gene expression, and hsa-miR-19b-3p. Receiver Operating Characteristic (ROC) curve analysis revealed robust diagnostic potential, with area under the curve (AUC) values of 0.71, 0.73, and 0.76 for serum IL-1R1, hsa-miR-19b-3p, and IL-1R1 gene expression, respectively. Elevated IL-1R1 and hsa-miR-19b-3p levels are associated with AA and its severity, suggesting these markers have potential as diagnostic and prognostic indicators. These findings enhance the understanding of IL-1R1's role in AA and highlight potential molecular targets for future therapeutic approaches.
斑秃（AA）是一种以脱发为特征的自身免疫性疾病，与涉及白细胞介素-1受体1型（IL-1R1）途径的炎症过程相关。本研究旨在探讨IL-1R1基因表达、血清IL-1R1水平和hsa-miR-19b-3p与AA严重程度之间的关系。采用病例对照设计，我们评估了100例AA患者和100例健康对照，通过酶联免疫吸附测定（ELISA）测量血清IL-1R1，并通过定量实时PCR（qRT-PCR）分析IL-1R1基因和hsa-miR-19b-3p的表达水平。生物信息学分析预测hsa-miR-19b-3p在IL-1R1基因上有一个结合位点，表明该微小RNA在AA病理过程中具有调节作用。结果显示，与对照组相比，AA患者的血清IL-1R1、IL-1R1基因表达和hsa-miR-19b-3p水平显著更高。在AA队列中，严重病例的水平最高，血清IL-1R1、IL-1R1基因表达和hsa-miR-19b-3p之间存在显著相关性。受试者工作特征（ROC）曲线分析显示出强大的诊断潜力，血清IL-1R1、hsa-miR-19b-3p和IL-1R1基因表达的曲线下面积（AUC）值分别为0.71、0.73和0.76。IL-1R1和hsa-miR-19b-3p水平升高与AA及其严重程度相关，表明这些标志物有作为诊断和预后指标的潜力。这些发现加深了对IL-1R1在AA中作用的理解，并突出了未来治疗方法的潜在分子靶点。
