Hill D E, Fetterer R H, Romanowski R D, Urban J F
United States Department of Agriculture, Beltsville Agricultural Research Center-East, MD 20705-2350.
Vet Immunol Immunopathol. 1994 Aug;42(2):161-9. doi: 10.1016/0165-2427(94)90005-1.
The development of immunity to Ascaris suum was studied in pigs immunized with isolated cuticle fragments from A. suum second and third stage larvae (L2/L3) and adult worms, and compared with other methods that stimulate a strong protective response in pigs. A significant protective response was seen in animals immunized with isolated cuticle fragments from A. suum L2/L3 and adults, but it was less than that seen in animals inoculated with UV-irradiated eggs or naturally exposed to eggs on a dirt lot. Significant IgG responses to 2-mercaptoethanol (2ME)-soluble cuticle components were seen in all groups, but the level of the antibody response did not relate to protection. Group differences in antibody and lymphocyte blastogenic responses to cuticle proteins indicated quantitative and qualitative stage specific differences in 2ME-soluble and insoluble cuticular proteins. Intestinal immunity was notably absent from cuticle immunized pigs because a marked liver white spot response was observed following the challenge inoculation. Thus, cuticle fragments from larval and adult A. suum are capable of inducing a protective response to larval migration; however, the development of intestinal immunity is not a direct function of exposure to these antigens.
对用猪蛔虫第二和第三期幼虫（L2/L3）及成虫的分离角质层片段免疫的猪的猪蛔虫免疫发育进行了研究，并与在猪中刺激强烈保护反应的其他方法进行了比较。在用猪蛔虫L2/L3和成虫的分离角质层片段免疫的动物中观察到了显著的保护反应，但低于接种紫外线照射卵或在脏地上自然接触卵的动物中所见的保护反应。在所有组中均观察到对2-巯基乙醇（2ME）可溶性角质层成分的显著IgG反应，但抗体反应水平与保护无关。对角质层蛋白的抗体和淋巴细胞增殖反应的组间差异表明，2ME可溶性和不溶性角质层蛋白在数量和质量上存在阶段特异性差异。角质层免疫的猪明显缺乏肠道免疫，因为在攻毒接种后观察到明显的肝脏白斑反应。因此，猪蛔虫幼虫和成虫的角质层片段能够诱导对幼虫移行的保护反应；然而，肠道免疫的发展并非直接取决于这些抗原的暴露。
