Kovaleva O V, Podlesnaya P A, Kudinova E S, Sinyov V V, Mochalnikova V V, Kushlinskii N E, Gratchev A N
N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2025 Mar;178(5):626-630. doi: 10.1007/s10517-025-06387-x. Epub 2025 Apr 28.
The expression of long non-coding RNAs (lncRNAs) PSMB8-AS1 and IGFL2-AS1, as well as associated microRNAs miR-802 and miR-382-3p, was analyzed in 42 paired samples of non-small cell lung cancer (NSCLC) with subsequent evaluation of their diagnostic and prognostic significance. The expression of PSMB8-AS1 was reduced in tumor tissue compared to normal lung tissue, while the expression levels of IGFL2-AS1, miR-802, and miR-382-3p were increased. The analysis of the clinical significance of the studied molecules showed that the expression of PSMB8-AS1 correlated with the histological type and localization of the tumor (r = -0.532, p = 0.0003, and r = -0.475, p = 0.003, respectively). ROC analysis for PSMB8-AS1 in the group of patients with squamous cell carcinoma showed that the area under the ROC curve was 0.904 (p < 0.0001), indicating good quality of the diagnostic model and allows us to use the data obtained to further verify it. The analysis of the prognostic significance has shown that the studied non-coding RNAs in general are not prognostic factors for NSCLC. Additional subgroup analysis showed that PSMB8-AS1 is an unfavorable prognostic factor for squamous cell lung cancer (hazard ratio (HR)= 4.482, p = 0.027). Further investigation of non-coding RNAs in NSCLC is promising for identifying new markers for the diagnosis and prognosis of this disease.
在42对非小细胞肺癌（NSCLC）样本中分析了长链非编码RNA（lncRNA）PSMB8-AS1和IGFL2-AS1以及相关微小RNA miR-802和miR-382-3p的表达情况，随后评估了它们的诊断和预后意义。与正常肺组织相比，肿瘤组织中PSMB8-AS1的表达降低，而IGFL2-AS1、miR-802和miR-382-3p的表达水平升高。对所研究分子的临床意义分析表明，PSMB8-AS1的表达与肿瘤的组织学类型和定位相关（分别为r = -0.532，p = 0.0003和r = -0.475，p = 0.003）。对鳞状细胞癌患者组中PSMB8-AS1的ROC分析表明，ROC曲线下面积为0.904（p < 0.0001），表明诊断模型质量良好，使我们能够利用所得数据进一步验证它。预后意义分析表明，所研究的非编码RNA总体上不是NSCLC的预后因素。额外的亚组分析表明，PSMB8-AS1是肺鳞状细胞癌的不良预后因素（风险比（HR）= 4.482，p = 0.027）。对NSCLC中非编码RNA的进一步研究有望为该疾病的诊断和预后鉴定新的标志物。
