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长链非编码RNA PSMA3-AS1通过海绵化miR-4504促进肺癌生长和侵袭。

LncRNA PSMA3-AS1 Promotes Lung Cancer Growth and Invasion via Sponging MiR-4504.

作者信息

Li Fangfang, Yu LianLing, Zhu Jun

机构信息

Department of Respiration Medicine, Qingdao Eighth People's Hospital, Qingdao 266000, People's Republic of China.

Department of Inspection, The 5th People's Hospital of Jinan, Jinan 250022, People's Republic of China.

出版信息

Cancer Manag Res. 2020 Jul 1;12:5277-5283. doi: 10.2147/CMAR.S253575. eCollection 2020.

DOI:10.2147/CMAR.S253575
PMID:32669876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7335846/
Abstract

BACKGROUND

Long noncoding RNAs (lncRNAs) have close correlation with tumorigenesis. And how lncRNAs participate in lung cancer require investigation in-depth. The aim of this study was to determine the role of lncRNA PSMA3-AS1 in lung cancer progression.

METHODS

PSMA3-AS1 expression was analyzed via qRT-PCR. Kaplan-Meier method was used to analyze survival rate based on PSMA3-AS1 value. Proliferation was measured via CCK8 and colony formation assays. Transwell assay was utilized to examine migration and invasion. Luciferase reporter assay and RNA pulldown assay were utilized to analyze the interaction between PSMA3-AS1 and miR-4504.

RESULTS

PSMA3-AS1 expression was upregulated in lung cancer tissues and cell lines. PSMA3-AS1 expression was positively correlated with clinical stage and metastasis. PSMA3-AS1 overexpression predicted a poor prognosis in lung cancer patients. PSMA3-AS1 knockdown suppressed proliferation, migration and invasion of lung cancer cells. Through bioinformatics analysis, PSMA3-AS1 was predicted to sponge miR-4504. MiR-4504 expression was inhibited by PSMA3-AS1. And inhibition of miR-4504 reversed the effects of PSMA3-AS1 depletion.

CONCLUSION

PSMA3-AS1 promotes the tumorigenesis of lung cancer through inhibiting miR-4504.

摘要

背景

长链非编码RNA（lncRNA）与肿瘤发生密切相关。lncRNA如何参与肺癌发生仍需深入研究。本研究旨在确定lncRNA PSMA3-AS1在肺癌进展中的作用。

方法

通过qRT-PCR分析PSMA3-AS1表达。采用Kaplan-Meier法基于PSMA3-AS1值分析生存率。通过CCK8和集落形成试验检测增殖。利用Transwell试验检测迁移和侵袭。采用荧光素酶报告基因试验和RNA下拉试验分析PSMA3-AS1与miR-4504之间的相互作用。

结果

PSMA3-AS1在肺癌组织和细胞系中表达上调。PSMA3-AS1表达与临床分期和转移呈正相关。PSMA3-AS1过表达预示肺癌患者预后不良。敲低PSMA3-AS1可抑制肺癌细胞的增殖、迁移和侵袭。通过生物信息学分析，预测PSMA3-AS1可吸附miR-4504。PSMA3-AS1抑制miR-4504表达。抑制miR-4504可逆转PSMA3-AS1缺失的作用。

结论

PSMA3-AS1通过抑制miR-4504促进肺癌的肿瘤发生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9288/7335846/6e4b4d0a4bbc/CMAR-12-5277-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9288/7335846/1df4ac9db717/CMAR-12-5277-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9288/7335846/bc7401ff7cec/CMAR-12-5277-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9288/7335846/af68623fce5c/CMAR-12-5277-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9288/7335846/6e4b4d0a4bbc/CMAR-12-5277-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9288/7335846/1df4ac9db717/CMAR-12-5277-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9288/7335846/bc7401ff7cec/CMAR-12-5277-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9288/7335846/af68623fce5c/CMAR-12-5277-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9288/7335846/6e4b4d0a4bbc/CMAR-12-5277-g0004.jpg

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