This study aimed to identify the effects of major metal components present in particulate matter (PM)2.5 on the reproductive system, sperm function, and embryo development. Through intratracheal instillation, male mice were exposed to various concentrations of metal components, including calcium oxide (Ca), iron oxide (Fe), aluminum oxide (Al), zinc oxide (Zn), lead oxide (Pb), and a mixture of these metals, in PM2.5 collected from the porcine farm. After 14 days, testicular inflammation and abnormal sperm morphology were observed in the exposed mice. These results indicate that such metal exposure enhances inflammatory cytokines in the testis and oxidative stress-induced apoptosis. Moreover, the exposure influenced sperm deformation, capacitation status, testosterone levels, and testosterone biosynthesis. Importantly, embryo development was also found to be impacted due to decreased sperm fertility. This study demonstrates that major metal components of PM2.5 derived from porcine farm pose adverse effects on the male reproductive system.