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利用交叉遗传学对特定起源的血管平滑肌细胞进行精准靶向的新工具。

New Tools for Precision Targeting of Origin-Specific Vascular Smooth Muscle Cells Using Intersectional Genetics.

作者信息

Majesky Mark W

机构信息

Center for Developmental Biology & Regenerative Medicine, Seattle Children's Research Institute, Seattle, WA. Heart Center, Division of Cardiology, Seattle Children's Hospital, Seattle, WA. Department of Pediatrics, University of Washington School of Medicine, Seattle, WA, 98105.

出版信息

Circulation. 2025 Apr 29;151(17):1268-1271. doi: 10.1161/CIRCULATIONAHA.125.073364. Epub 2025 Apr 28.

DOI:10.1161/CIRCULATIONAHA.125.073364
PMID:40294144
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12110322/
Abstract

Intersectional genetics is an advanced dual recombinase technology developed to gain more precision in cell fate mapping and gene function analysis . It relies on overlapping expression patterns of a pair of site-specific DNA recombinases, Cre and Dre, to gain high-resolution targeting of specific cell populations. In this issue of , Han et al report the application of intersectional genetics to target four SMC subtypes residing in different segments of disease-prone arteries. The innovative new tools generated and the precision targeting results obtained suggest a powerful new experimental approach is available to test the possibility that the regional distribution of vascular disease has a developmental basis .

摘要

交叉遗传学是一种先进的双重组酶技术，旨在提高细胞命运图谱绘制和基因功能分析的精度。它依赖于一对位点特异性DNA重组酶Cre和Dre的重叠表达模式，以实现对特定细胞群体的高分辨率靶向。在本期杂志中，Han等人报告了交叉遗传学在靶向易患疾病动脉不同节段的四种平滑肌细胞亚型中的应用。所产生的创新新工具和获得的精确靶向结果表明，有了一种强大的新实验方法来检验血管疾病的区域分布是否具有发育基础这一可能性。

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New Tools for Precision Targeting of Origin-Specific Vascular Smooth Muscle Cells Using Intersectional Genetics.利用交叉遗传学对特定起源的血管平滑肌细胞进行精准靶向的新工具。
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2
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本文引用的文献

1
Exploring Origin-Dependent Susceptibility of Smooth Muscle Cells to Aortic Diseases Through Intersectional Genetics.通过交叉遗传学探索平滑肌细胞对主动脉疾病的起源依赖性易感性。
Circulation. 2025 Apr 29;151(17):1248-1267. doi: 10.1161/CIRCULATIONAHA.124.070782. Epub 2025 Feb 10.
2
Generation and Comparative Analysis of an Mouse with Preferential Activity in Vascular Smooth Muscle Cells.一种在血管平滑肌细胞中具有优先活性的小鼠的构建及比较分析。
Nat Cardiovasc Res. 2022 Nov;1(11):1084-1100. doi: 10.1038/s44161-022-00162-1. Epub 2022 Nov 11.
3
Promoters to Study Vascular Smooth Muscle.研究血管平滑肌的启动子。
Arterioscler Thromb Vasc Biol. 2019 Apr;39(4):603-612. doi: 10.1161/ATVBAHA.119.312449.
4
Lineage tracking of origin and fate of smooth muscle cells in atherosclerosis.动脉粥样硬化中平滑肌细胞起源和命运的谱系追踪。
Cardiovasc Res. 2018 Mar 15;114(4):492-500. doi: 10.1093/cvr/cvx251.
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Enhancing the precision of genetic lineage tracing using dual recombinases.使用双重组酶提高遗传谱系追踪的精度。
Nat Med. 2017 Dec;23(12):1488-1498. doi: 10.1038/nm.4437. Epub 2017 Nov 13.
6
Smooth Muscle Cells Derived From Second Heart Field and Cardiac Neural Crest Reside in Spatially Distinct Domains in the Media of the Ascending Aorta-Brief Report.源自第二心脏场和心脏神经嵴的平滑肌细胞位于升主动脉中膜的不同空间区域——简要报告
Arterioscler Thromb Vasc Biol. 2017 Sep;37(9):1722-1726. doi: 10.1161/ATVBAHA.117.309599. Epub 2017 Jun 29.
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The earliest metanephric arteriolar progenitors and their role in kidney vascular development.最早的后肾血管祖细胞及其在肾脏血管发育中的作用。
Am J Physiol Regul Integr Comp Physiol. 2015 Jan 15;308(2):R138-49. doi: 10.1152/ajpregu.00428.2014. Epub 2014 Nov 26.
8
Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice.Dre 重组酶与 Cre 一样，是大肠杆菌、哺乳动物细胞和小鼠中一种高效的位点特异性重组酶。
Dis Model Mech. 2009 Sep-Oct;2(9-10):508-15. doi: 10.1242/dmm.003087. Epub 2009 Aug 19.
9
Developmental origin of smooth muscle cells in the descending aorta in mice.小鼠降主动脉中平滑肌细胞的发育起源
Development. 2008 May;135(10):1823-32. doi: 10.1242/dev.020958. Epub 2008 Apr 16.
10
Sclerotomal origin of vascular smooth muscle cells and pericytes in the embryo.胚胎中血管平滑肌细胞和周细胞的巩膜节起源。
Dev Biol. 2008 Mar 15;315(2):437-47. doi: 10.1016/j.ydbio.2007.12.045. Epub 2008 Jan 11.