Monocytes play a pivotal role in the immune regulation of labor, contributing to processes like cervical ripening, uterine contractility, and the initiation of parturition. During labor, monocytes are recruited to the cervix and uterus, where they undergo activation and release pro-inflammatory cytokines that help mediate uterine contractions and facilitate cervical remodeling. However, immune dysregulation involving excessive or insufficient monocyte activation can contribute to complications such as prolonged labor. The imbalance in cytokine production and monocyte dysfunction is thought to be a key factor in delayed labor, making monocytes critical targets for understanding and managing labor abnormalities. Recent studies have explored the potential of monocyte-related biomarkers as predictive tools for identifying women at risk for prolonged labor. Monocyte subsets and cytokine profiles, including markers such as CD14, CD16, interleukin-1β, tumor necrosis factor-alpha, and interleukin-6, offer valuable insights into the inflammatory status of the cervix and uterus. The ability to assess monocyte function and cytokine levels may provide early indications of immune dysregulation, allowing for timely interventions to prevent prolonged labor and reduce complications. Advances in diagnostic technologies, such as cytokine assays and flow cytometry, are improving our ability to monitor monocyte activity during labor in real time.