Network pharmacology of the regulation of Th17/Treg cell immune balance in the pathogenesis of arthritis by the combination of Chuanxiong Rhizoma, Radix Angelicae Biseratae and Achyranthis Bidentatae Radix.

This study explores the network pharmacological mechanism of the regulatory effect of Chuanxiong Rhizoma, Radix Angelicae Biseratae and Achyranthis Bidentatae Radix combination on Th17/Treg cell immune balance in the pathogenesis of arthritis. Databases such as the Chinese Pharmacopoeia, TCMSP, and BATMAN-TCM were used to obtain the active ingredients of Chuanxiong Rhizoma, Radix Angelicae Biseratae and Achyranthis Bidentatae Radix. After screening and weight removal, the target of action was determined using the TCMSP database. GeneCards and OMIN databases were used to screen the relevant targets for arthritis; A drug active ingredient-arthritis-target network model was constructed using Cytoscape and further analyze it; Protein-protein interaction networks were built using STRING database; R4.0.2 software was used to complete gene ontology analysis of intersection targets and enrichment of Kyoto Gene and Gene Encyclopedia pathway. After screening and weight removal, a total of 14 effective chemical components were obtained, corresponding to a total of 340 targets. After screening and weight removal, 171 active component targets were ultimately obtained. Transforming protein-proteins into gene names resulted in a total of 75 targets, which were used as candidate target sources for prescription. The drug targets of Chuanxiong Rhizoma, Radix Angelicae Biseratae and Achyranthis Bidentatae Radix, and arthritis were imported into R software, and 41 targets were obtained with relevant tools; Using the CytoNCA plug-in to complete the PPT further topology, PTGS2, AKT1, CASP3, CAT, and SOD1 are the top 5 nodes in terms of degree values; gene ontology enrichment analysis obtained 1332 entries, including 1120 biological process entries, 112 cell composition entries, and 100 molecular functions. Chuanxiong Rhizoma, Radix Angelicae Biseratae and Achyranthis Bidentatae Radix in combination can produce positive effects on the pathophysiology of arthritis and can enhance the synergistic effects of several medications. The immunological balance of Th17/Treg cells may have a regulatory role in the pathway.