Han Cong, Chen Jing, Shen Chuanlin, Liang Qiuxia, An Ying, Zhou Chaoyi, Liu Kechun, Xia Qing, He Qiuxia, Zhang Huazheng
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, People's Republic of China.
Department of Tibetan Medicine, Tibetan Traditional Medicine College, Lhasa, Tibet, People's Republic of China.
Drug Des Devel Ther. 2025 Apr 24;19:3159-3177. doi: 10.2147/DDDT.S505880. eCollection 2025.
Shiyiwei Golden Pill (SYW) is a classic traditional prescription used to treat mKhris-pa according to the theory of Tibetan medicine. At present, SYW is widely used to treat cholecystitis in Tibetan areas. However, the chemical constituents and anti-inflammatory mechanisms are still largely undiscovered. This study aimed to investigate the chemical composition and anti-inflammatory effects of SYW, as well as its potential mechanisms.
The components of SYW were identified using ultrahigh-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The anti-inflammatory effects of SYW were determined on zebrafish and RAW264.7 cell inflammation models. Additionally, we predicted the targets and pathways of SYW to confirm its anti-inflammatory effects using network pharmacology approaches. Finally, a quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the expression of genes associated with anti-inflammatory pathways.
We identified 94 compounds in SYW, mainly alkaloids, phenols, and flavonoids. SYW inhibited inflammatory cell proliferation and migration in the three zebrafish inflammation models. In the RAW264.7 cell model, SYW suppressed the levels of NO and pro-inflammatory cytokines. In addition, network pharmacology analysis revealed that ALB, IL6, TNF, AKT1, and EGFR were identified as the potential key targets of SYW. KEGG enrichment and qRT-PCR analysis showed that PI3K/Akt/FoxO signaling pathway was involved in the anti-inflammatory effects of SYW.
Herein, we identified 94 chemical constituents of SYW and demonstrated that SYW exerts anti-inflammatory effects by regulating the PI3K/Akt/FoxO signaling pathway.
十一味金色丸（SYW）是藏医理论中用于治疗赤巴病的经典传统方剂。目前，SYW在藏区广泛用于治疗胆囊炎。然而，其化学成分和抗炎机制仍大多未被发现。本研究旨在探讨SYW的化学成分、抗炎作用及其潜在机制。
采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-Q-TOF/MS）鉴定SYW的成分。在斑马鱼和RAW264.7细胞炎症模型上测定SYW的抗炎作用。此外，我们使用网络药理学方法预测SYW的靶点和通路以证实其抗炎作用。最后，进行定量实时聚合酶链反应（qRT-PCR）以验证与抗炎通路相关基因的表达。
我们在SYW中鉴定出94种化合物，主要为生物碱、酚类和黄酮类。SYW在三种斑马鱼炎症模型中抑制炎症细胞增殖和迁移。在RAW264.7细胞模型中，SYW抑制NO和促炎细胞因子水平。此外，网络药理学分析显示ALB、IL6、TNF、AKT1和EGFR被确定为SYW的潜在关键靶点。KEGG富集和qRT-PCR分析表明PI3K/Akt/FoxO信号通路参与了SYW的抗炎作用。
在此，我们鉴定出SYW的94种化学成分，并证明SYW通过调节PI3K/Akt/FoxO信号通路发挥抗炎作用。
