Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Nicolas Cabrera 1, Cantoblanco, 28049 Madrid, Spain.
J Exp Bot. 2021 Oct 13;72(19):6708-6715. doi: 10.1093/jxb/erab303.
Organogenesis in plants is primarily postembryonic and relies on a strict balance between cell division and cell expansion. The root is a particularly well-suited model to study cell proliferation in detail since the two processes are spatially and temporally separated for all the different tissues. In addition, the root is amenable to detailed microscopic analysis to identify cells progressing through the cell cycle. While it is clear that cell proliferation activity is restricted to the root apical meristem (RAM), understanding cell proliferation kinetics and identifying its parameters have required much effort over many years. Here, we review the main concepts, experimental settings, and findings aimed at obtaining a detailed knowledge of how cells proliferate within the RAM. The combination of novel tools, experimental strategies, and mathematical models has contributed to our current view of cell proliferation in the RAM. We also discuss several lines of research that need to be explored in the future.
植物的器官发生主要是在胚胎后期进行的，依赖于细胞分裂和细胞扩张之间的严格平衡。根是一个特别适合的模型，可以详细研究细胞增殖，因为所有不同的组织在空间和时间上都将这两个过程分离开来。此外，根还适合进行详细的微观分析，以确定正在通过细胞周期的细胞。虽然很明显，细胞增殖活性仅限于根顶端分生组织（RAM），但多年来，为了了解细胞增殖动力学并确定其参数，人们付出了很多努力。在这里，我们回顾了主要的概念、实验设置和发现，旨在获得关于细胞在 RAM 内增殖的详细知识。新工具、实验策略和数学模型的结合，促进了我们目前对 RAM 中细胞增殖的看法。我们还讨论了未来需要探索的几个研究方向。
