Danielides Gerasimos, Lygeros Spyridon, Kyriakopoulos Georgios, Tsapardoni Foteini, Grafanaki Katerina, Stathopoulos Constantinos, Naxakis Stephanos, Danielides Vassileios
Department of Otorhinolaryngology, Medical School, University of Patras, Patras, Greece.
Department of Biochemistry, Medical School, University of Patras, Patras, Greece.
Am J Rhinol Allergy. 2025 Jul;39(4):259-265. doi: 10.1177/19458924251332546. Epub 2025 Apr 29.
BackgroundThere is increasing evidence of the contribution of periostin in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP).ObjectiveThis study aims to investigate the expression of periostin in nasal polyp tissue along with serum periostin and its potential function as a biomarker.MethodsThe study included 36 CRSwNP patients and 12 controls. Nasal polyp tissue from CRSwNP patients and inferior turbinate mucosa samples from controls were intraoperatively collected. gene mRNA expression from nasal polyp tissue was assessed with polymerase chain reaction and periostin levels on tissue samples were measured with ELISA and Western Blot. ELISA was performed to evaluate serum periostin on blood samples collected from patients undergoing functional endoscopic sinus surgery for CRSwNP ( = 36) and results were compared with assays performed on control subjects ( = 12).ResultsTissue periostin gene expression in terms of mRNA levels was significantly elevated in patients with CRSwNP than in healthy control subjects (-value <.05). Τissue periostin values in CRSwNP patients were also significantly higher in CRSwNP compared to controls. A positive correlation between periostin levels and the presence of asthma and smoking was observed in the patient group. Serum periostin did not present a statistically significant difference between the 2 groups.ConclusionsOur data suggest that periostin expression is upregulated in nasal polyps and might determine a crucial role in the occurrence and evolution of the disease. Periostin appears to be a promising therapeutic target candidate in CRSwNP.
越来越多的证据表明骨膜蛋白在伴鼻息肉的慢性鼻-鼻窦炎（CRSwNP）发病机制中发挥作用。
本研究旨在探讨骨膜蛋白在鼻息肉组织中的表达情况以及血清骨膜蛋白水平，并研究其作为生物标志物的潜在功能。
本研究纳入36例CRSwNP患者和12例对照。术中收集CRSwNP患者的鼻息肉组织和对照的下鼻甲黏膜样本。采用聚合酶链反应评估鼻息肉组织中骨膜蛋白基因mRNA表达，并用酶联免疫吸附测定（ELISA）和蛋白质免疫印迹法检测组织样本中的骨膜蛋白水平。对因CRSwNP接受功能性鼻内镜鼻窦手术的患者（n = 36）采集的血样进行ELISA检测以评估血清骨膜蛋白，并将结果与对对照受试者（n = 12）进行的检测结果进行比较。
CRSwNP患者组织骨膜蛋白基因在mRNA水平的表达显著高于健康对照受试者（P值<0.05）。CRSwNP患者的组织骨膜蛋白值也显著高于对照组。在患者组中观察到骨膜蛋白水平与哮喘和吸烟之间存在正相关。两组之间血清骨膜蛋白无统计学显著差异。
我们的数据表明，骨膜蛋白在鼻息肉中表达上调，可能在疾病的发生和发展中起关键作用。骨膜蛋白似乎是CRSwNP中一个有前景的治疗靶点候选物。
