Salami Wasiu Olawale, Ajoseh Samuel Oluwasegun, Lawal-Sanni Aminat Olajumoke, El Tawab Ashraf A Abd, Neubauer Heinrich, Wareth Gamal, Akinyemi Kabiru Olusegun
Department of Microbiology, Faculty of Science, Lagos State University, Ojo P.O. Box 0001 LASU Post Office, Lagos 102101, Nigeria.
Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Toukh 13511, Egypt.
Antibiotics (Basel). 2025 Apr 12;14(4):398. doi: 10.3390/antibiotics14040398.
: species present significant health risks due to their widespread presence in humans, animals, and the environment. This study examined the patterns of antimicrobial resistance (AMR) and the presence of carbapenemase-producing species from various sources. : Between November 2023 and February 2024, 500 samples were collected in Lagos State, including 350 clinical human samples, 50 environmental samples, and 100 animal samples. The samples were processed, and isolates were identified and subjected to antimicrobial susceptibility tests (AST) by standard methods. Furthermore, carbapenemase ( and ) and virulence genes () were detected by real-time polymerase chain reaction (RT-PCR) methods using specific primers. : The overall prevalence of isolates was 4.6% (23/500), including 18 and 5 . The source prevalence was 24% (12/50) from the environmental samples, 5% (5/100) from animal sources, and 1.7% (6/350) from the clinical samples. All isolates were 100% resistant to ciprofloxacin, erythromycin, imipenem, vancomycin, and ampicillin. However, 91% were susceptible to gentamicin. Six (6) distinct resistance profiles were observed, with the pattern AMP-ERY-TGC-CIP-TS-VA-CHL-AUG-MEM-IMI being the most frequent in 12 (4 isolates from humans, 2 from animals, and 6 from the environment). Notably, 39.1% (9/23) of multiple-drug resistant isolates harbored the virulence gene, including seven (five environmental and two human) and two from animal sources. The strains HB003 and HB050, from human bacteremia cases carrying , were the first in Nigeria to produce and carbapenemase genes. : This study revealed the emergence of carbapenemase-producing species in our environment. A one-health approach and further molecular studies are essential to mitigate the spread and understand the transmission dynamics.
某些物种因其在人类、动物和环境中的广泛存在而带来重大健康风险。本研究调查了来自各种来源的抗菌药物耐药性（AMR）模式以及产碳青霉烯酶物种的存在情况。：在2023年11月至2024年2月期间，在拉各斯州收集了500份样本，包括350份临床人类样本、50份环境样本和100份动物样本。对样本进行处理，通过标准方法鉴定分离株并进行抗菌药物敏感性试验（AST）。此外，使用特异性引物通过实时聚合酶链反应（RT-PCR）方法检测碳青霉烯酶（和）以及毒力基因（）。：分离株的总体流行率为4.6%（23/500），包括18株和5株。环境样本中的来源流行率为24%（12/50），动物来源为5%（5/100），临床样本为1.7%（6/350）。所有分离株对环丙沙星、红霉素、亚胺培南、万古霉素和氨苄西林的耐药率均为100%。然而，91%对庆大霉素敏感。观察到六种不同的耐药谱，其中AMP-ERY-TGC-CIP-TS-VA-CHL-AUG-MEM-IMI模式在12株中最为常见（4株来自人类，2株来自动物，6株来自环境）。值得注意的是，39.1%（9/23）的多重耐药分离株携带毒力基因，包括7株（5株来自环境，2株来自人类）和2株来自动物来源。来自携带的人类菌血症病例的菌株HB003和HB050是尼日利亚首次产生和碳青霉烯酶基因的菌株。：本研究揭示了我们环境中产碳青霉烯酶物种的出现。采取“同一健康”方法并开展进一步的分子研究对于减轻传播并了解传播动态至关重要。
