Lin Po-An, Huang Hsiang-Han, Hu Mei-Hua, Huang Go-Shine, Meng En, Chiu Yi-Lin, Hsu Yung-Chi, Chan Wei-Hung
Department of Anesthesiology, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, Taiwan.
Division of Pediatric General Medicine, Department of Pediatrics, Chang Gung Memorial Hospital at LinKou, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.
Biomedicines. 2025 Apr 21;13(4):1006. doi: 10.3390/biomedicines13041006.
: This study aimed to investigate the effects of prostate tissue on platelet activation markers, primarily assessed through P-selectin expression, and to assess the formation of platelet-leukocyte aggregations in response to prostate tissue exposure. Furthermore, we compared platelet activation induced by prostate tissue homogenates with that induced by thrombin stimulation. These processes may play a role in the development of disseminated intravascular coagulation (DIC) following transurethral resection of the prostate (TURP). : We collected prostate tissue samples from 12 patients undergoing TURP. The samples were homogenized and used to stimulate platelet-rich plasma in vitro. Flow cytometry was used to measure platelet P-selectin expression and platelet-leukocyte aggregation. Additionally, four experimental groups were established: (A) saline control, (B) thrombin stimulation, (C) phosphate-buffered saline (PBS) control, and (D) prostate tissue homogenate. Data were analyzed to assess the impact of prostate tissue and thrombin on platelet activation and platelet-leukocyte interactions. : Prostate tissue homogenates significantly increased platelet P-selectin expression and platelet-neutrophil aggregation compared with the control groups ( < 0.05). Overall, platelet-leukocyte aggregation was not significantly different between the thrombin and prostate tissue groups. However, prostate tissue exposure did not significantly affect platelet-monocyte and platelet-lymphocyte aggregations. : Prostate tissue exposure during TURP induces platelet activation, particularly platelet P-selectin expression and platelet-neutrophil aggregation, suggesting a potential mechanism for DIC development. These findings highlight the importance of monitoring platelet activity in patients undergoing TURP and indicate that interventions targeting platelet P-selectin expression and platelet-neutrophil interactions may help mitigate DIC risk.
本研究旨在调查前列腺组织对血小板活化标志物的影响（主要通过P-选择素表达进行评估），并评估暴露于前列腺组织后血小板-白细胞聚集体的形成。此外，我们比较了前列腺组织匀浆诱导的血小板活化与凝血酶刺激诱导的血小板活化。这些过程可能在经尿道前列腺电切术（TURP）后弥散性血管内凝血（DIC）的发生中起作用。
我们从12例接受TURP的患者中收集前列腺组织样本。将样本匀浆并用于体外刺激富含血小板的血浆。采用流式细胞术测量血小板P-选择素表达和血小板-白细胞聚集。此外，设立了四个实验组：（A）生理盐水对照组、（B）凝血酶刺激组、（C）磷酸盐缓冲盐水（PBS）对照组和（D）前列腺组织匀浆组。对数据进行分析，以评估前列腺组织和凝血酶对血小板活化及血小板-白细胞相互作用的影响。
与对照组相比，前列腺组织匀浆显著增加了血小板P-选择素表达和血小板-中性粒细胞聚集（<0.05）。总体而言，凝血酶组和前列腺组织组之间的血小板-白细胞聚集无显著差异。然而，暴露于前列腺组织并未显著影响血小板-单核细胞和血小板-淋巴细胞聚集。
TURP期间暴露于前列腺组织会诱导血小板活化，尤其是血小板P-选择素表达和血小板-中性粒细胞聚集，提示了DIC发生的潜在机制。这些发现凸显了监测接受TURP患者血小板活性的重要性，并表明针对血小板P-选择素表达和血小板-中性粒细胞相互作用的干预措施可能有助于降低DIC风险。
