Gao Junying, Yu Lindong, Qi Haoran, Qi Jun, Zheng Zhaodi
Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, China.
Department of Spine Surgery, Spinal Deformity Center, The Second Xiangya Hospital of Central South University, Changsha, China.
Genesis. 2025 Apr;63(2):e70013. doi: 10.1002/dvg.70013.
Single-cell RNA sequencing (scRNA-seq) is a rapidly developing and useful technique for elucidating biological mechanisms and characterizing individual cells. Tens of millions of patients worldwide suffer from heart injuries and other types of heart disease. Neonatal mammalian hearts and certain adult vertebrate species, such as zebrafish, can fully regenerate after myocardial injury. However, the adult mammalian heart is unable to regenerate the damaged myocardium. scRNA-seq provides many new insights into pathological and normal hearts and facilitates our understanding of cellular responses to cardiac injury and repair at different stages, which may provide critical clues for effective therapies for adult heart regeneration. In this review, we summarize the application of scRNA-seq in heart development and regeneration and describe how important molecular mechanisms can be harnessed to promote heart regeneration.
单细胞RNA测序（scRNA-seq）是一种快速发展且有用的技术，用于阐明生物学机制和表征单个细胞。全球数以千万计的患者患有心脏损伤和其他类型的心脏病。新生哺乳动物心脏以及某些成年脊椎动物物种，如斑马鱼，在心肌损伤后能够完全再生。然而，成年哺乳动物心脏无法再生受损的心肌。scRNA-seq为病理性和正常心脏提供了许多新见解，并有助于我们理解不同阶段细胞对心脏损伤和修复的反应，这可能为成人心脏再生的有效治疗提供关键线索。在这篇综述中，我们总结了scRNA-seq在心脏发育和再生中的应用，并描述了如何利用重要的分子机制来促进心脏再生。
