Werling H O, Spiess E, Aulenbacher P, Paweletz N
Invasion Metastasis. 1985;5(5):270-94.
The rat tumor cell system BSp73AS/BSp73ASML was investigated for its structural aspects. The non-metastasizing line BSp73AS has less nuclear atypism than the metastasizing line BSp73ASML. Microvilli are scanty and variable in length and structure; in BSp73ASML they cover the cells densely, are short and homogeneous in size. In vitro adhesion and spreading tests show structurally flexible BSp73AS cells which flatten completely onto substrata. BSp73ASML cells remain spherical and develop only small attachment areas. In vitro aggregation of the BSp73AS cells leads to round, tightly packed aggregates, their outer cell layer is epitheloid BSp73ASML cells form clusters of mostly ball-shaped cells. Surprisingly, as judged from EM images, the intercellular junctions in BSp73ASML cell clusters are qualitatively the same as in BSp73AS cell aggregates. However, the extent of apposition between cells in BSp73AS aggregates is much higher than between cells in the BSp73ASML clusters. BSp73ASML cells lack the ability to vary their shape. This is the prominent difference revealed by these tests between the two cell lines. Hence, this fact could play an important role in their different metastatic behavior in vivo. We speculate that the ability to vary the cell shape is necessary for expansive growth in the host environment but not for the metastatic spread.
对大鼠肿瘤细胞系BSp73AS/BSp73ASML的结构方面进行了研究。非转移性细胞系BSp73AS的核异型性比转移性细胞系BSp73ASML少。微绒毛稀少，长度和结构各异；在BSp73ASML中，它们密集覆盖细胞，短小且大小均匀。体外黏附和铺展试验表明，结构灵活的BSp73AS细胞能完全扁平铺展在基质上。BSp73ASML细胞保持球形，仅形成小的附着区域。BSp73AS细胞的体外聚集导致形成圆形、紧密堆积的聚集体，其外层细胞呈上皮样，而BSp73ASML细胞形成大多为球形细胞的簇。令人惊讶的是，从电子显微镜图像判断，BSp73ASML细胞簇中的细胞间连接在性质上与BSp73AS细胞聚集体中的相同。然而，BSp73AS聚集体中细胞间的贴附程度远高于BSp73ASML簇中的细胞间贴附程度。BSp73ASML细胞缺乏改变其形状的能力。这是这些试验揭示的两个细胞系之间的显著差异。因此，这一事实可能在它们在体内不同的转移行为中起重要作用。我们推测，改变细胞形状的能力对于在宿主环境中的扩张性生长是必要的，但对于转移扩散并非必要。
