Paweletz N, Werling H O, Aulenbacher P, Spiess E
Scan Electron Microsc. 1984(Pt 2):783-92.
The morphological characteristics of two rat tumor cell lines were studied by means of scanning electron microscopy. While the ASML-cells (Ascites, Solid, Metastases, Lung) when spread via the lymphatic vessels form metastases in the lung, the AS-cells (Ascites, Solid) do not form secondary tumors. The ASML-cells do not attach on glass or plastic but grow in suspension; AS-cells, can be cultivated as monolayers. The ASML-cells form loosely packed clusters when they are shaken in a gyratory shaker, AS-cells produce well organized aggregates in which the intercellular contacts are very intensive. When a mixture of cells from both lines is aggregated the two types sort out and the line-specific aggregate or cluster is formed without interaction between the two types. ASML-cells remain spherical when confronted with pieces of the aortic wall and there are no signs of reaction with the underlying endothelial cells. The endothelium is drastically altered when AS-cells are seeded on top of the aortic wall: the tumor cells flatten, penetrate the endothelial layer and induce the endothelial cells to retract. These phenomena can also be seen in confrontations with the mesothelium of the diaphragm. As also cells from aggregates or clusters behave as the single cells we conclude that the newly formed heterotypic contacts are favored over homotypic ones. In addition they have proved to be more intensive.
通过扫描电子显微镜研究了两种大鼠肿瘤细胞系的形态特征。ASML细胞（腹水、实体、转移、肺）通过淋巴管扩散时会在肺部形成转移灶，而AS细胞（腹水、实体）不会形成继发性肿瘤。ASML细胞不附着在玻璃或塑料上，而是悬浮生长；AS细胞可以作为单层培养。ASML细胞在旋转振荡器中摇晃时形成松散堆积的聚集体，AS细胞产生组织良好的聚集体，其中细胞间接触非常紧密。当两种细胞系的细胞混合物聚集时，两种类型会分开，形成特定细胞系的聚集体或簇，两种类型之间没有相互作用。当ASML细胞与主动脉壁碎片接触时仍保持球形，没有与下层内皮细胞反应的迹象。当AS细胞接种在主动脉壁顶部时，内皮会发生剧烈变化：肿瘤细胞扁平，穿透内皮层并诱导内皮细胞回缩。在与膈肌间皮的接触中也能看到这些现象。由于聚集体或簇中的细胞行为与单个细胞相同，我们得出结论，新形成的异型接触比同型接触更受青睐。此外，它们已被证明更为紧密。
