Alagha Heba Z, Gülsoy Murat
Institute of Biomedical Engineering, Boğaziçi University, Istanbul, Turkey.
Recent Adv Antiinfect Drug Discov. 2025;20(1):64-76. doi: 10.2174/0127724344309438240529064221.
The aim of this study was to investigate the antimicrobial photodynamic, sonodynamic, and combined photodynamic and sonodynamic potentials of IR780 iodide loaded mesoporous silica nanoparticles against gram-negative () and multi drug resistant (MDR ).
IR780 iodide loaded mesoporous silica nanoparticles were synthesized, and their antimicrobial photodynamic and sonodynamic potentials against one strain, and one MDR strain were investigated. Laser irradiation was achieved via a 785 nm diode laser (500 mW/cm, 5 min). Ultrasound irradiation was achieved via a 1-MHz ultrasound unit (1.5 W/cm, 50% duty cycle, 3 min). Viable bacterial cells were counted by serial dilution method. Data were analyzed by ANOVA followed by Tukey's test (p ≤ 0.05).
The results revealed that for , the combined photodynamic therapy (PDT) and sonodynamic therapy (SDT) showed a 44% reduction in bacterial cell viability as compared to 18% and 31% when exposed to SDT alone and PDT alone, respectively. For MDR. , the combined treatment resulted in a 45% reduction in bacterial cell viability, as compared to 14% and 30% when exposed to SDT alone and PDT alone, respectively. The killing effect was mainly due to the photodynamic and sonodynamic effects of the nanoparticles, mainly caused by singlet oxygen. No photothermal effect was involved in the killing.
The results of this study demonstrated that IR780 iodide-loaded mesoporous silica nanoparticles have the potential to be utilized as photo/sono therapeutic agents for the inactivation of drug-resistant bacteria.
本研究旨在探究负载碘化 IR780 的介孔二氧化硅纳米颗粒对革兰氏阴性菌（）和多重耐药菌（MDR）的抗菌光动力、声动力及联合光动力和声动力的潜力。
合成了负载碘化 IR780 的介孔二氧化硅纳米颗粒，并研究了其对一株菌和一株 MDR 菌的抗菌光动力和声动力潜力。通过 785 nm 二极管激光器（500 mW/cm²，5 分钟）进行激光照射。通过 1-MHz 超声单元（1.5 W/cm²，50%占空比，3 分钟）进行超声照射。采用系列稀释法对活菌细胞进行计数。数据采用方差分析，随后进行 Tukey 检验（p≤0.05）。
结果显示，对于，联合光动力疗法（PDT）和声动力疗法（SDT）使细菌细胞活力降低了 44%，而单独暴露于 SDT 和 PDT 时分别降低了 18%和 31%。对于 MDR，联合治疗使细菌细胞活力降低了 45%，而单独暴露于 SDT 和 PDT 时分别降低了 14%和 30%。杀伤作用主要归因于纳米颗粒的光动力和声动力效应，主要由单线态氧引起。杀伤过程中未涉及光热效应。
本研究结果表明，负载碘化 IR780 的介孔二氧化硅纳米颗粒有潜力用作光/声治疗剂来灭活耐药细菌。
