Zhou Zheng, Duan Yonghao, Li Yajing, Zhang Pan, Li Qing, Yu Luyao, Han Cuicui, Huo Juncheng, Chen Wansheng, Xiao Ying
State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong New Area, Shanghai 201203, China.
Navy Special Medical Centre, Second Military Medical University, 800 Xiangyin Road, Yangpu District, Shanghai 200433, China.
Hortic Res. 2025 Mar 10;12(6):uhaf074. doi: 10.1093/hr/uhaf074. eCollection 2025 Jun.
Phenolic compounds are derived from the phenylpropanoid metabolic pathways of plants and include phenylpropionic acids, lignins, coumarins, and flavonoids. These compounds are among the most abundant and diverse classes of secondary metabolites found throughout the plant kingdom. Phenolic compounds play critical roles in the growth, development, and stress resistance of horticultural plants. Moreover, some phenolic compounds exhibit substantial biological activities, and they are widely utilized across various sectors, such as the pharmaceutical and food industries. The cytochrome P450 monooxygenase 98A subfamily (CYP98A) is involved mainly in the biosynthesis of phenolic compounds, mediating the -hydroxylation of aromatic rings in the common phenylpropane biosynthesis pathways of phenolic compounds. However, research on this family of oxidases is currently fragmented, and a systematic and comprehensive review has not yet been conducted. This review offers an exhaustive summary of the molecular features of the CYP98A family and the functions of CYP98A monooxygenases in the biosynthesis of different types of phenolic compounds. In addition, this study provides a reference for the exploration and functional study of plant CYP98A family enzymes. An enhanced understanding of CYP98A monooxygenases can help in the cultivation of high-quality horticultural plants with increased resistance to biotic and abiotic stresses and enhanced accumulation of natural bioactive compounds via metabolic engineering strategies. Moreover, the structural optimization and modification of CYP98A monooxygenases can provide additional potential targets for synthetic biology, enabling the efficient production of important phenolic compounds to address production supply conflicts.
酚类化合物源自植物的苯丙烷代谢途径，包括苯丙酸、木质素、香豆素和黄酮类化合物。这些化合物是植物界中最丰富、种类最多样的次生代谢产物之一。酚类化合物在园艺植物的生长、发育和抗逆性中发挥着关键作用。此外，一些酚类化合物具有显著的生物活性，在制药和食品工业等各个领域都有广泛应用。细胞色素P450单加氧酶98A亚家族（CYP98A）主要参与酚类化合物的生物合成，在酚类化合物常见的苯丙烷生物合成途径中介导芳香环的羟基化反应。然而，目前关于该氧化酶家族的研究较为零散，尚未进行系统全面的综述。本综述详尽总结了CYP98A家族的分子特征以及CYP98A单加氧酶在不同类型酚类化合物生物合成中的功能。此外，本研究为植物CYP98A家族酶的探索和功能研究提供了参考。深入了解CYP98A单加氧酶有助于通过代谢工程策略培育出对生物和非生物胁迫具有更强抗性且天然生物活性化合物积累量增加的高品质园艺植物。此外，对CYP98A单加氧酶进行结构优化和修饰可为合成生物学提供更多潜在靶点，从而高效生产重要酚类化合物以解决生产供应矛盾。
