Petráš Jiří, Bártová Eva, Žákovská Alena
Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Bohunice, Brno, Czech Republic.
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palackého tř. 1946/1, 612 42, Brno, Czech Republic.
Transbound Emerg Dis. 2023 Oct 17;2023:2511753. doi: 10.1155/2023/2511753. eCollection 2023.
sensu lato (sl) is spirochetes transmitted by ticks and known to cause Lyme disease. -like organisms (CLOs) comprise a large group of bacteria that can lead to serious health disorders, including miscarriage. Recently, CLOs have been found in ticks and patient skin biopsies. Due to the involvement of multiple potential vectors in the spread of these pathogens, the objective of this study was to confirm the presence of both organisms in the early developmental stages of selected vectors. Three potential vectors, larvae, larvae, and winged (unfed) adults of , were collected in the Czech Republic in years 2019-2020. The presence of sl and panchlamydial DNA was detected by PCR and positive samples were further analyzed by Sanger sequencing and phylogenetic tree construction. sl DNA was proved in 1.5% (2/137) of larvae (identified as and ), in 1.7% (2/119) of larvae (both identified as ), and in 11% (3/27) of (all identified as ). CLOs were identified in 0.7% (1/137) of larvae (Candidatus ) and in 7.4% (2/27) of (unspecified genus), while larvae could not be evaluated (0%). This research represents the first investigation of the presence of CLOs in . The detection of pathogen DNA in the early developmental stages of vectors suggests the potential for transgenerational transmission of sl and CLOs in the selected vectors, although at a low rate.
广义（sensu lato，sl）是由蜱传播的螺旋体，已知可引起莱姆病。类衣原体样生物（CLOs）是一大类细菌，可导致严重的健康问题，包括流产。最近，在蜱和患者皮肤活检样本中发现了CLOs。由于多种潜在媒介参与了这些病原体的传播，本研究的目的是确认所选媒介早期发育阶段中这两种生物的存在。2019 - 2020年在捷克共和国采集了三种潜在媒介，即 幼虫、 幼虫和 有翅（未进食）成虫。通过PCR检测sl和泛衣原体DNA的存在，并对阳性样本进一步进行桑格测序和系统发育树构建。在1.5%（2/137）的 幼虫（鉴定为 和 ）中检测到sl DNA，在1.7%（2/119）的 幼虫（均鉴定为 ）中检测到sl DNA，在11%（3/27）的 （均鉴定为 ）中检测到sl DNA。在0.7%（1/137）的 幼虫（暂定种 ）和7.4%（2/27）的 （未指定属）中鉴定出CLOs，而 幼虫无法进行评估（0%）。本研究是对 中CLOs存在情况的首次调查。在媒介早期发育阶段检测到病原体DNA表明，sl和CLOs在所选媒介中存在跨代传播的可能性，尽管传播率较低。
