Zhou Xingdong, Bian Sushu, Kan Enxi, Zhou Lujia, Zhang Xiaohui, Xiao Min, Lu Chang, Hua Ji, Wu Yuan, Zhang Cheng, Zhou Yingshan, Dong Wanyu, Du Jing, Wang Xiaodu, Song Houhui
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China.
Jiangxi Zhengbang Academy of Agricultural Sciences, Nanchang 330029, China.
Transbound Emerg Dis. 2023 Jun 30;2023:4001055. doi: 10.1155/2023/4001055. eCollection 2023.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen causing substantial financial losses in the global swine industry. The prevention of PRRSV is hampered due to frequent gene recombination among different strains of PRRSV. In this study, a new PRRSV strain, PRRSV-HQ-2020, was identified from nursery piglets in Yunnan Province, China, in 2020. The complete genome analysis revealed that PRRSV-HQ-2020 is highly similar to JXA1-like (lineage 8.7 PRRSV, isolated from China in 2008) in the 5'UTR, nsp1-9, and nsp11 coding regions. Additionally, it has a resemblance to JM (lineage 3 PRRSV, isolated from Taiwan, China, in 2010) in the nsp12-M coding region and NADC30 (lineage 1.8 PRRSV, isolated from North American in 2008) in the nsp10, N, and 3'UTR, suggesting a natural recombination event. Furthermore, recombination analyses showed three interlineage recombination events among lineages 8.7, 1.8, and 3. Notably, the GP5 protein of PRRSV-HQ-2020 exhibited a crucial mutation at position 44, leading to the deletion of a key glycosylation site. These findings provide direct evidence for the natural occurrence of recombination events among three lineages of PRRSV-2 in Chinese swine herds, leading to the emergence of unique genetic properties of PRRSV variants, and providing a theoretical basis for developing better PRRSV prevention strategies.
猪繁殖与呼吸综合征病毒（PRRSV）是一种重要病原体，在全球养猪业中造成巨大经济损失。由于PRRSV不同毒株之间频繁发生基因重组，其预防工作受到阻碍。在本研究中，2020年从中国云南省的保育仔猪中鉴定出一种新的PRRSV毒株PRRSV-HQ-2020。全基因组分析表明，PRRSV-HQ-2020在5'UTR、nsp1-9和nsp11编码区与JXA1-like（2008年从中国分离的8.7谱系PRRSV）高度相似。此外，它在nsp12-M编码区与JM（2010年从中国台湾分离的3谱系PRRSV）相似，在nsp10、N和3'UTR与NADC30（2008年从北美分离的1.8谱系PRRSV）相似，表明发生了自然重组事件。此外，重组分析显示在8.7、1.8和3谱系之间存在三次谱系间重组事件。值得注意的是，PRRSV-HQ-2020的GP5蛋白在第44位发生了关键突变，导致一个关键糖基化位点缺失。这些发现为中国猪群中PRRSV-2的三个谱系之间自然发生重组事件提供了直接证据，导致PRRSV变体出现独特的遗传特性，并为制定更好的PRRSV预防策略提供了理论依据。
