Frederiks W M, Marx F
Histochem J. 1985 Jun;17(6):707-15. doi: 10.1007/BF01003522.
The tetrazolium method for the histochemical detection of monoamine oxidase (MAO) activity in rat liver cryostat sections has been tested for its specificity and its possible use in quantification. The tetrazolium salt tetranitro blue tetrazolium is recommended for the localization of MAO activity, rather than nitro blue tetrazolium or BPST [2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium] . Hardly any formazan was produced in the absence of the substrate tryptamine and Marsilid, a specific inhibitor of MAO activity, prevented formazan production almost completely. A linear relationship between the integrated absorbance measured with a microdensitometer and either the incubation period or section thickness was obtained. We conclude that the method described in this paper can be used for the quantitative analysis of MAO activity in tissue sections of rat liver. MAO activity was found to be 20-25% higher in the periportal zone of rat liver than in the perivenous zone.
已对用于大鼠肝脏恒冷箱切片中单胺氧化酶（MAO）活性组织化学检测的四氮唑法进行了特异性测试及其在定量分析中的潜在用途测试。推荐使用四氮唑盐四硝基蓝四氮唑来定位MAO活性，而不是硝基蓝四氮唑或BPST [2-(2-苯并噻唑基)-3-(4-邻苯二甲酰肼基)-5-苯乙烯基-四氮唑]。在没有底物色胺的情况下几乎不产生甲臜，并且MAO活性的特异性抑制剂异烟酰异丙肼几乎完全阻止了甲臜的产生。用显微密度计测量的积分吸光度与孵育时间或切片厚度之间获得了线性关系。我们得出结论，本文所述方法可用于大鼠肝脏组织切片中MAO活性的定量分析。发现大鼠肝脏门周区的MAO活性比肝静脉周围区高20 - 25%。
