Van Noorden C J, Tas J, Vogels I M
Histochem J. 1983 Jun;15(6):583-99. doi: 10.1007/BF01954149.
With the aid of thin films of polyacrylamide gel containing purified glucose-6-phosphate dehydrogenase subjected to cytochemical procedures for the enzyme using tetranitro blue tetrazolium, arbitrary units of integrated absorbance obtained with a Barr & Stroud GN5 cytophotometer were converted into units of enzyme activity. This conversion enabled cytochemical data to be compared directly with biochemical values. The conversion was applied to the cytochemical estimation of glucose-6-phosphate dehydrogenase activity in isolated rat hepatocytes, mouse oocytes, rabbit thymocytes, human granulocytes and human fibroblasts. Several control procedures were performed to confirm the admissibility of this conversion, such as: the estimation of the absorption characteristics of the formazans of tetranitro blue tetrazolium both in solution and precipitated in biological specimens; the linearity of the relationship between the increase of absorbance and incubation time; and the effect of different incubation conditions on the amount of specific formazan production.
借助含有纯化葡萄糖-6-磷酸脱氢酶的聚丙烯酰胺凝胶薄膜，使用四硝基蓝四唑对该酶进行细胞化学检测，用巴尔与斯特劳德GN5型细胞光度计获得的积分吸光度任意单位被转换为酶活性单位。这种转换使得细胞化学数据能够直接与生化值进行比较。该转换应用于分离的大鼠肝细胞、小鼠卵母细胞、兔胸腺细胞、人粒细胞和人成纤维细胞中葡萄糖-6-磷酸脱氢酶活性的细胞化学测定。进行了若干对照实验以确认这种转换的可接受性，例如：测定四硝基蓝四唑甲臜在溶液中和沉淀于生物标本中的吸收特性；吸光度增加与孵育时间之间关系的线性；以及不同孵育条件对特定甲臜生成量的影响。
