Záborszky L, Léránth C
Histochemistry. 1985;82(6):529-37. doi: 10.1007/BF00489974.
In order to study the synaptic connections of neurons identified by their projection target and neurotransmitter content, we have adapted a method of combining retrograde tracing of horseradish peroxidase (HRP) and immunocytochemistry at the electron microscopic level. HRP was injected into the rat amygdala. Sections from the rostral forebrain were processed according to the 3,3'-diaminobenzidine/glucose oxidase reaction followed by choline acetyltransferase (ChAT) localization. Neurons in the ventral pallidum which contained both the diffuse immunoperoxidase reaction product (ChAT) and large electron dense bodies characteristic of retrogradely transported HRP were defined as double labeled, i.e. cholinergic neurons that project to the amygdaloid body.
为了研究根据投射靶点和神经递质含量确定的神经元的突触连接，我们采用了一种在电子显微镜水平上结合辣根过氧化物酶（HRP）逆行追踪和免疫细胞化学的方法。将HRP注入大鼠杏仁核。根据3,3'-二氨基联苯胺/葡萄糖氧化酶反应处理前脑嘴侧的切片，随后进行胆碱乙酰转移酶（ChAT）定位。腹侧苍白球中既含有弥散性免疫过氧化物酶反应产物（ChAT）又含有逆行运输的HRP特征性大电子致密体的神经元被定义为双重标记，即投射到杏仁体的胆碱能神经元。
