Drescher B, Lauke H, Hartmann M, Davidoff M S, Zumkeller W
Department of Anatomy, University Hospital Eppendorf, Germany.
Mol Pathol. 1997 Dec;50(6):298-303. doi: 10.1136/mp.50.6.298.
To study the immunohistochemical localisation of insulin-like growth factor (IGF) I, IGF II, and IGF binding proteins 1-6 in intratubular germ cell neoplasia in the vicinity of solid germ cell tumours of the testis.
Testes were obtained from 13 patients (20-35 years old) who had undergone orchidectomy for treatment of a solid germ cell tumour. Tumour cells were verified histologically by their distinctive morphology and by visualisation of placental alkaline phosphatase immunoreactivity.
The majority of carcinoma in situ (CIS) cells were immunopositive for IGF I, whereas no CIS cells stained for IGF II. Of all the IGF binding proteins investigated, CIS cells showed intense immunoreactivity for IGF binding protein 5 and lower expression of all other IGF binding proteins.
These results suggest that the action of IGF binding protein 5 in CIS cells may modulate the activity of IGF I. This may be related to a proliferative advantage that could facilitate tumour development.
研究胰岛素样生长因子（IGF）I、IGF II以及IGF结合蛋白1 - 6在睾丸实体生殖细胞肿瘤附近的管内生殖细胞瘤变中的免疫组织化学定位。
从13例（20 - 35岁）因治疗实体生殖细胞肿瘤而接受睾丸切除术的患者获取睾丸。通过肿瘤细胞独特的形态学以及胎盘碱性磷酸酶免疫反应性的可视化对肿瘤细胞进行组织学验证。
大多数原位癌（CIS）细胞对IGF I呈免疫阳性，而没有CIS细胞对IGF II染色。在所有研究的IGF结合蛋白中，CIS细胞对IGF结合蛋白5显示出强烈的免疫反应性，而对所有其他IGF结合蛋白的表达较低。
这些结果表明IGF结合蛋白5在CIS细胞中的作用可能调节IGF I的活性。这可能与一种增殖优势有关，这种优势可能促进肿瘤发展。
