Rosenkrans W A, Penney D P
Int J Radiat Oncol Biol Phys. 1985 Sep;11(9):1629-37. doi: 10.1016/0360-3016(85)90215-9.
The thoraces of male LAF1 mice were irradiated at doses of 5, 9, or 13 Gy. The animals were killed at times of 1 hr, 1 day, 1 wk, 4 wks, and 12 wks postirradiation (PI). The lungs were removed, enzymatically or detergent digested, fixed with ruthenium red for demonstration of anionic sites, and processed for electron microscopy. Untreated (0 Gy, 0 time) and sham irradiated control groups were also processed. Sections of lungs were examined and changes in alveolar basal laminar anionic sites were quantitated. Changes in three groups of glycosaminoglycans (GAGs) were identified: chondroitin 4 and 6 sulfate-dermatan sulfate, hyaluronate, and other GAGs (principally heparan sulfate). At 1 hr PI, all groups showed a marked decrease in site number over controls, which continued to 1 wk. By 4 wks there was a marked relative increase in heparan sulfate containing sites for doses of 13 Gy and a moderate increase for the other doses. At 12 wks the level of heparan sulfate was considerably above normal for doses of 13 Gy and just above normal for the other doses. Chondroitin-dermatan sulfate had recovered by 12 wks for doses of 13 Gy, but was still subnormal for other doses; however, hyaluronate-containing sites recovered only slightly by 12 wks. The implications for this change on basal laminar permeability and the induction of fibrosis are discussed.
对雄性LAF1小鼠的胸部进行5、9或13 Gy剂量的照射。在照射后（PI）1小时、1天、1周、4周和12周时处死动物。取出肺脏，进行酶消化或去污剂消化，用钌红固定以显示阴离子位点，然后进行电子显微镜处理。未处理（0 Gy，0时间）和假照射对照组也进行处理。检查肺切片并对肺泡基底层阴离子位点的变化进行定量。确定了三组糖胺聚糖（GAGs）的变化：硫酸软骨素4和6-硫酸皮肤素、透明质酸和其他GAGs（主要是硫酸乙酰肝素）。在照射后1小时，所有组的位点数量均比对照组显著减少，这种情况持续到1周。到4周时，对于13 Gy剂量，含硫酸乙酰肝素的位点有显著相对增加，其他剂量有适度增加。在12周时，对于13 Gy剂量，硫酸乙酰肝素水平大大高于正常，其他剂量略高于正常。对于13 Gy剂量，硫酸软骨素-硫酸皮肤素在12周时已恢复，但其他剂量仍低于正常；然而，含透明质酸的位点在12周时仅略有恢复。讨论了这种变化对基底层通透性和纤维化诱导的影响。
