Penney D P, Rosenkrans W A
Radiat Res. 1984 Aug;99(2):410-9.
The lungs of male LAF1/J mice were locally irradiated with graded doses of radiation ranging from 5 to 13 Gy. The animals were sacrificed at 1 hr, 1 day, 1 week, 4 weeks, and 12 weeks postirradiation (PI), fixed with Ruthenium Red/Triton X-100 for demonstration of basal laminar anionic sites, and processed for electron microscopy. Sham-irradiated (0 Gy, 0 time) and untreated control groups were also processed. Sections of lungs were examined ultrastructurally and changes in both alveolar and capillary basal laminar anionic sites quantitated. A marked decrease in the number of basal laminar anionic sites was noted 1 hr PI in both alveolar and capillary basal laminae at all dose levels. The decline continued to 1 week for doses of 13 Gy and more gradually to 4 weeks following doses of 5 and 9 Gy, when the number of sites began to increase. By 12 weeks animals receiving 13 Gy were approaching normal levels while those receiving 5 or 9 Gy remained subnormal. The potential effects of the loss of proteoglycans with radiation on lung basal laminar permeability and cell-extracellular matrix interactions are discussed.
对雄性LAF1/J小鼠的肺部进行局部照射，辐射剂量从5 Gy到13 Gy不等。在照射后1小时、1天、1周、4周和12周处死动物，用钌红/ Triton X - 100固定以显示基膜阴离子位点，并进行电子显微镜处理。也对假照射（0 Gy，0时间）和未处理的对照组进行处理。对肺组织切片进行超微结构检查，并对肺泡和毛细血管基膜阴离子位点的变化进行定量分析。在所有剂量水平下，照射后1小时，肺泡和毛细血管基膜中基膜阴离子位点的数量均显著减少。对于13 Gy的剂量，这种下降持续到1周，而对于5 Gy和9 Gy的剂量，下降则较为缓慢，到4周时位点数量开始增加。到12周时，接受13 Gy照射的动物接近正常水平，而接受5 Gy或9 Gy照射的动物仍低于正常水平。讨论了辐射导致蛋白聚糖丢失对肺基膜通透性和细胞-细胞外基质相互作用的潜在影响。
