Wang Hong, Niu Pilian, Li Haishan, Feng Lu, Li Zhenkai, Bai Mingsheng, Peng Li
School of Life Sciences, Ningxia University, Yinchuan, 750021, China.
College of Resource and Environment and Life Science, Ningxia Normal University, Guyuan, 756000, China.
Plant Foods Hum Nutr. 2025 Apr 30;80(2):117. doi: 10.1007/s11130-025-01343-6.
Myocardial fibrosis is the main pathological feature of cardiovascular diseases. Stellariae Radix polysaccharide has many pharmacological effects, but its anti-cardiac fibrosis activity has not been reported. In this study that the structure of a new polysaccharide SRP-1 from Stellariae Radix was identified, and its anti-myocardial fibrosis effect was discussed. SRP-1 was extracted from Stellariae Radix and purified by DEAE-52 cellulose chromatography. According to physicochemical methods and monosaccharide composition analysis, SRP-1 was found to be mainly composed of galactose, glucose, xylose, fructose, mannose, and rhamnose, and its weight average molecular weight (Mw) was 31,309 Da. Tests of activity against myocardial fibrosis indicated marked downregulation of the expression of Col I, Col III, α-SMA, and proteins related to the TGF-β/Smad signaling pathway, which were induced by TGF-β1, in mouse cardiac fibroblasts pretreated with SRP-1. In addition, SRP-1restrained the abnormal growth of cardiac fibroblasts cells and the expression of Snail 1, Snail 2, Twist 1, and Slug mRNA in vitro. These results indicate that Stellariae Radix polysaccharide may warrant further analysis as a novel therapeutic agent for cardiovascular diseases.
心肌纤维化是心血管疾病的主要病理特征。银柴胡多糖具有多种药理作用，但其抗心脏纤维化活性尚未见报道。在本研究中，鉴定了一种来自银柴胡的新型多糖SRP-1的结构，并探讨了其抗心肌纤维化作用。SRP-1从银柴胡中提取，并用DEAE-52纤维素柱色谱法纯化。根据理化方法和单糖组成分析，发现SRP-1主要由半乳糖、葡萄糖、木糖、果糖、甘露糖和鼠李糖组成，其重均分子量（Mw）为31,309 Da。对心肌纤维化的活性测试表明，在用SRP-1预处理的小鼠心脏成纤维细胞中，由TGF-β1诱导的Col I、Col III、α-SMA以及与TGF-β/Smad信号通路相关的蛋白质的表达明显下调。此外，SRP-1在体外抑制了心脏成纤维细胞的异常生长以及Snail 1、Snail 2、Twist 1和Slug mRNA的表达。这些结果表明，银柴胡多糖作为一种新型的心血管疾病治疗药物可能值得进一步研究。
