College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, 26 Yuxiang Street, Shijiazhuang, 050018, China.
College of Pharmacy, Hebei University of Chinese Medicine, No. 3 Xingyuan Road, Shijiazhuang, 050200, China.
Plant Foods Hum Nutr. 2023 Jun;78(2):390-398. doi: 10.1007/s11130-023-01070-w. Epub 2023 May 30.
An acidic polysaccharide (SMP) with a molecular weight (Mw) of 1.28 × 10 Da was isolated from Salvia miltiorrhiza. The monosaccharide composition in molar percentages was rhamnose (Rha): galacturonic acid (GalA): galactose (Gal): arabinose (Ara) = 6.15: 55.98: 21.27: 16.69. The results of simulated digestion in vitro showed that SMP was not degraded in saliva, gastric juice or intestinal juice. The Y maze test and new object recognition test showed that SMP could improve the working memory impairment of aging mice. SMP could also increase the activity of superoxide dismutase (SOD) and catalase (CAT) in serum and brain tissue, decrease the content of malondialdehyde (MDA), decrease the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in brain tissue, and increase the content of short-chain fatty acids (SCFA) in the intestine. In addition, SMP could also regulate the intestinal flora structure, including increasing the relative abundance of Firmicutes and Bacteroidetes and decreasing the relative abundance of Proteobacteria. This work lays a foundation for the development of functional foods related to Salvia miltiorrhiza.
从丹参中分离得到一种相对分子质量（Mw）为 1.28×10 Da 的酸性多糖（SMP）。单糖组成摩尔百分比为鼠李糖（Rha）：半乳糖醛酸（GalA）：半乳糖（Gal）：阿拉伯糖（Ara）=6.15:55.98:21.27:16.69。体外模拟消化结果表明，SMP 唾液、胃液和肠液中均不被降解。Y 迷宫试验和新物体识别试验表明，SMP 可改善衰老小鼠的工作记忆障碍。SMP 还可提高血清和脑组织中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性，降低丙二醛（MDA）的含量，降低脑组织中白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平，并增加肠道短链脂肪酸（SCFA）的含量。此外，SMP 还可以调节肠道菌群结构，包括增加厚壁菌门和拟杆菌门的相对丰度，降低变形菌门的相对丰度。这项工作为开发与丹参相关的功能性食品奠定了基础。
