Chua Aleesa E, Go Eden P, Desaire Heather
Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA.
Biomolecules. 2025 Mar 25;15(4):477. doi: 10.3390/biom15040477.
In large-scale studies, uncontrolled systematic variability introduced during sample preparation, processing, and storage can interfere with the detection of subtle biological signals. This study evaluates storage conditions, including two sample preparation methods and storage durations, to minimize systematic variability in the analysis of extracted lipids from latent fingerprints. In the traditional approach, samples are prepared immediately, stored as lipid extracts, and processed in multiple batches. In an alternative method, samples are stored directly on the deposition foil, and preparation is delayed until all can be processed in a single batch. Storage duration is evaluated to determine if shorter storage with analysis in multiple batches is more effective than longer storage with analysis in a single batch. Our findings demonstrate that storage of latent fingerprint samples on the deposition foil is a viable option, with minimal degradation of key features even after eight months of storage. While some differences in lipid profiles were observed across storage conditions, these differences were minor and would likely have little impact in larger studies where biological variability is greater. These insights offer practical guidance for implementing latent fingerprint sampling in large-scale studies by identifying optimal conditions that preserve sample quality and streamline workflows.
在大规模研究中，样品制备、处理和储存过程中引入的未控制的系统变异性可能会干扰细微生物信号的检测。本研究评估了储存条件，包括两种样品制备方法和储存时长，以尽量减少从潜指纹中提取脂质分析时的系统变异性。在传统方法中，样品立即制备，作为脂质提取物储存，并分批处理。在另一种方法中，样品直接储存在沉积箔上，制备工作推迟到所有样品可以一次性处理。评估储存时长以确定分批分析时较短的储存时间是否比较长的储存时间和一次性分析更有效。我们的研究结果表明，将潜指纹样品储存在沉积箔上是一个可行的选择，即使储存八个月后关键特征的降解也最小。虽然在不同储存条件下观察到脂质谱存在一些差异，但这些差异很小，在生物变异性更大的大规模研究中可能影响不大。这些见解通过确定保持样品质量和简化工作流程的最佳条件，为大规模研究中实施潜指纹采样提供了实用指导。
