Scherberich Jan, Bauer Leopold, Huber-Liu Nana, Krombach Gabriele Anja, Metzger Roman, Kluth Dietrich, Ginzel Marco
Department of Diagnostic and Interventional Radiology (Experimental Radiology), University Hospital Giessen, 35392 Giessen, Germany.
Department of Pediatric and Adolescent Surgery, Paracelsus Medical University Hospital, 5020 Salzburg, Austria.
Biomolecules. 2025 Apr 11;15(4):569. doi: 10.3390/biom15040569.
Adriamycin, a chemotherapy drug, was first shown to induce malformations in rat embryos in 1978, with subsequent studies focusing on the development of esophageal atresia. Recently, we examined early tracheal development in healthy rat embryos using microcomputed tomography, which is useful for visualizing the morphology during embryonic development. Here, we use this technique to show malformations in rat embryos after Adriamycin treatment in the form of an atlas.
Pregnant rats received Adriamycin [1.75 mg/kg body weight] intraperitoneally once daily from day 7 to 9. The embryos were harvested between the 16th and 21st embryonic day, fixed in Bouin's solution and subsequently dried in a critical point dryer. The dried embryos were scanned using microcomputed tomography. Existing datasets of normal embryos served as controls.
The embryos affected by Adriamycin showed a broad spectrum of malformations that have only partially been described in previous publications. Our preliminary data show a large number of malformations that can be attributed to the VACTERL association (Vertebral, Anorectal, Cardiac, Tracheoesophageal, Renal, and Limb anomalies). In addition, cardiovascular malformations have been observed that may directly affect the esophageal/tracheal morphology.
Micro CT imaging used in Adriamycin-affected embryos shows the full spectrum of induced malformations.
阿霉素是一种化疗药物，1978年首次被证明可诱导大鼠胚胎出现畸形，随后的研究主要集中在食管闭锁的发育方面。最近，我们使用微计算机断层扫描技术检查了健康大鼠胚胎的早期气管发育情况，该技术有助于观察胚胎发育过程中的形态。在此，我们以图谱的形式使用该技术展示阿霉素处理后大鼠胚胎的畸形情况。
怀孕大鼠从第7天至第9天每天腹腔注射一次阿霉素[1.75毫克/千克体重]。在胚胎发育的第16天至第21天收集胚胎，固定于布因氏液中，随后在临界点干燥器中干燥。使用微计算机断层扫描对干燥后的胚胎进行扫描。正常胚胎的现有数据集用作对照。
受阿霉素影响的胚胎表现出广泛的畸形，此前的出版物仅部分描述过这些畸形。我们的初步数据显示，大量畸形可归因于VACTERL综合征（椎体、肛门直肠、心脏、气管食管、肾脏和肢体异常）。此外，还观察到心血管畸形，这些畸形可能直接影响食管/气管形态。
在受阿霉素影响的胚胎中使用微计算机断层扫描成像显示了所诱导畸形的全貌。
