Zhou Jie, Hou Hai-Tao, Chen Huan-Xin, Song Yu, Zhou Xiao-Lin, Zhang Li-Li, Xue Hong-Mei, Yang Qin, He Guo-Wei
Department of Cardiac Surgery & The Institute of Cardiovascular Diseases, TEDA International Cardiovascular Hospital, Tianjin University, Tianjin 300457, China.
Tianjin Key Laboratory of Molecular Regulation of Cardiovascular Diseases and Translational Medicine, Tianjin 300457, China.
Biomolecules. 2025 Apr 15;15(4):583. doi: 10.3390/biom15040583.
Myocardial infarction (MI), including ST-elevation MI (STEMI) and non-ST-elevation MI (NSTEMI), has been the leading cause of hospitalization and death. Exosomes participate in many physiological and pathological processes and have important effects on cell communication and function. This study analyzed the proteomic characteristics of plasma exosomes with the discovery of exosomal differentially expressed proteins (DEPs) in MI patients. Proteomics technology was used to identify the plasma exosomal DEPs in 41 patients in STEMI, NSTEMI, unstable angina, and CONTROL groups, and 406 exosomal DEPs were discovered. Further, 36 selected exosomal DEPs were validated with parallel reaction monitoring (PRM) in a new cohort of STEMI, NSTEMI, and CONTROL groups, and 7 were successfully verified. There were three (F13A1, TSPAN33, and YWHAZ) in the STEMI group and six (F13A1, TSPAN33, ITGA2B, GP9, GP5, and PPIA) in the NSTEMI group, and all were down-regulated compared to the CONTROL group with high sensitivity and specificity in MI that may be developed as biomarkers for MI and may become possible therapeutic targets for MI. Bioinformatics analysis revealed that these seven exosomal DEPs are of great significance in the molecular mechanism of MI. Therefore, the present study has provided insights to further explore the pathological mechanism and possible therapeutic targets in MI.
心肌梗死（MI），包括ST段抬高型心肌梗死（STEMI）和非ST段抬高型心肌梗死（NSTEMI），一直是住院和死亡的主要原因。外泌体参与许多生理和病理过程，对细胞通讯和功能有重要影响。本研究分析了MI患者血浆外泌体的蛋白质组学特征，发现了外泌体差异表达蛋白（DEP）。采用蛋白质组学技术鉴定STEMI、NSTEMI、不稳定型心绞痛和对照组41例患者的血浆外泌体DEP，共发现406个外泌体DEP。此外，在STEMI、NSTEMI和对照组的新队列中，用平行反应监测（PRM）验证了36个选定的外泌体DEP，其中7个得到成功验证。STEMI组有3个（F13A1、TSPAN33和YWHAZ），NSTEMI组有6个（F13A1、TSPAN33、ITGA2B、GP9、GP5和PPIA），与对照组相比均下调，在MI中具有高敏感性和特异性，可能发展为MI的生物标志物，并可能成为MI的潜在治疗靶点。生物信息学分析表明，这7个外泌体DEP在MI的分子机制中具有重要意义。因此，本研究为进一步探索MI的病理机制和可能的治疗靶点提供了思路。
