液相色谱法测定番茄及番茄制品中的细交链孢菌酮酸和链格孢酚甲醚

Liquid chromatographic determination of tenuazonic acid and alternariol methyl ether in tomatoes and tomato products.

作者信息

Stack M E, Mislivec P B, Roach J A, Pohland A E

出版信息

J Assoc Off Anal Chem. 1985 Jul-Aug;68(4):640-2.

Abstract

A liquid chromatographic (LC) method for determining tenuazonic acid (TA) and alternariol methyl ether (AME) in tomatoes and tomato products is described. The Alternaria metabolites are extracted from a water slurry of the sample with CHCl3, the mixture is centrifuged, and the extract is fractionated on a silica gel column. Reverse phase LC with an ultraviolet detector (for TA) and a fluorescence detector (for AME) connected in series is used for final separation and determination. The limit of determination for TA and AME is 25 and 3 ng/g, respectively, with average recoveries from catsup of 83 and 68%, respectively. The LC method also detects alternariol, but interfering peaks in some samples prevent accurate quantitation. Chemical ionization mass spectrometry (CIMS) is used to confirm TA. Samples (142) of tomatoes collected from commercial processing lines were analyzed; TA was found in 73 samples (0.4-70 micrograms/g).

摘要

本文描述了一种用于测定番茄及番茄制品中细交链孢菌酮酸（TA）和链格孢酚甲醚（AME）的液相色谱（LC）方法。将链格孢属代谢产物从样品的水浆中用CHCl₃萃取，混合物离心，提取物在硅胶柱上进行分馏。采用串联连接的紫外检测器（用于TA）和荧光检测器（用于AME）的反相LC进行最终分离和测定。TA和AME的测定限分别为25和3 ng/g，番茄酱中的平均回收率分别为83%和68%。该LC方法也能检测到链格孢酚，但某些样品中的干扰峰妨碍了准确定量。采用化学电离质谱（CIMS）来确证TA。分析了从商业加工生产线采集的142份番茄样品；在73份样品中发现了TA（0.4 - 70微克/克）。