Boese J, Nakashima M, Glaze L E
J Assoc Off Anal Chem. 1985 Jul-Aug;68(4):697-8.
Results are reported for a collaborative study of a method for the extraction of light filth from whole peppermint leaves. A 5 g sample is defatted with isopropanol in a simple reflux appartus. Rat hairs, insect fragments, and whole insects are isolated by wet sieving on a No. 230 sieve, a deaerating boil in 40% isopropanol solution, flotation with Tween 80-Na4EDTA (1 + 1) and mineral oil-heptane (85 + 15), and trappings in a Wildman trap flask. Average recoveries obtained by 6 collaborators for 3 spike levels of rat hairs (5, 10, 15) were 83.3, 87.5, and 82.2%, respectively. For whole insects (5, 10, 15) recoveries averaged 85.0, 80.0 and 77.2% respectively; for insect fragments (20, 30, 50) recoveries averaged 79.6, 88.3, and 84.8%, respectively. The average recoveries for the 3 levels of each analyte were not significantly different. The method has been adopted official first action.
本文报告了一项关于从薄荷叶中提取轻质杂质方法的合作研究结果。取5克样品，在简易回流装置中用异丙醇脱脂。通过在230号筛网上进行湿筛、在40%异丙醇溶液中进行脱气煮沸、用吐温80 - 四钠乙二胺（1 + 1）和矿物油 - 庚烷（85 + 15）进行浮选以及在威尔德曼捕集瓶中捕集，来分离大鼠毛发、昆虫碎片和完整昆虫。6位合作者对3个加标水平（5、10、15）的大鼠毛发的平均回收率分别为83.3%、87.5%和82.2%。对于完整昆虫（5、10、15），回收率平均分别为85.0%、80.0%和77.2%；对于昆虫碎片（20、30、50），回收率平均分别为79.6%、88.3%和84.8%。每种分析物3个水平的平均回收率无显著差异。该方法已被首次官方采用。
