Braine H G, Elfenbein G J, Mellits E D
J Clin Apher. 1985;2(3):213-8. doi: 10.1002/jca.2920020302.
Selected tests of lymphoid function were used to screen a population of volunteer hemapheresis donors. Testing included: 1) absolute lymphocyte numbers, and percentage of T-cell, B-cell, and mononuclear phagocytes, 2) serum immunoglobulins, and, 3) in vitro proliferative responses to lectin mitogens (phytohemagglutinin, concanavalin A, and pokeweed mitogen), soluble antigens (staphylococcal filtrate, candida, and streptococcal varidase), and cell-bound alloantigens (mixed lymphocyte culture). A control population of first-time plateletpheresis donors was examined similarly. Regular donors manifested a small but statistically significant decrease in absolute lymphocyte counts (p less than 0.02), and IgM (p less than 0.02) compared to controls. Leukapheresis donors also manifested significant decreases in percentage of T cells (p less than 0.02). These findings are qualitatively similar to changes reported following intensive lymphocytapheresis and indicate the need for conservative policies regarding donation frequency in hemapheresis programs.
使用选定的淋巴细胞功能测试对一群自愿的血液成分单采捐献者进行筛查。测试包括：1）淋巴细胞绝对计数以及T细胞、B细胞和单核吞噬细胞的百分比，2）血清免疫球蛋白，以及3）对凝集素促有丝分裂原（植物血凝素、刀豆球蛋白A和商陆促有丝分裂原）、可溶性抗原（葡萄球菌滤液、念珠菌和链球菌变应原酶）和细胞结合同种异体抗原（混合淋巴细胞培养）的体外增殖反应。对首次进行血小板单采捐献者的对照人群进行了类似检查。与对照组相比，定期捐献者的淋巴细胞绝对计数（p<0.02）和IgM（p<0.02）出现了虽小但具有统计学意义的下降。白细胞单采捐献者的T细胞百分比也出现了显著下降（p<0.02）。这些发现与强化淋巴细胞单采后报告的变化在性质上相似，表明在血液成分单采项目中需要制定关于捐献频率的保守政策。
