Takano T, Takigawa M, Suzuki F
J Biochem. 1985 Apr;97(4):1093-100. doi: 10.1093/oxfordjournals.jbchem.a135153.
Hydrocortisone stimulated glycosaminoglycan (GAG) synthesis, a characteristic of the cartilage phenotype, of rabbit costal chondrocytes in confluent quiescent culture, as judged by the incorporations of [35S]sulfate and [3H]glucosamine. Hydrocortisone also stimulated incorporation of [3H]serine into proteoglycan. The stimulation of GAG synthesis by hydrocortisone was dose-dependent and maximal at a physiological concentration of 10(-7) M. Hydrocortisone also stimulated GAG synthesis in cultures in the log-phase of growth. In this case, its maximal effect was observed at a concentration of 10(-6) M. The magnitude of the increase of GAG synthesis in response to hydrocortisone was larger in confluent culture than in log-phase cultures. Hydrocortisone stimulated DNA synthesis dose-dependently, and its effect was observable at a physiological concentration. However, no stimulation of DNA synthesis by hydrocortisone was observed in serum-free medium, in contrast to that of GAG synthesis. Hydrocortisone also increased protein synthesis and the cell number. Dexamethasone also stimulated the syntheses of both GAG and DNA. These results show that glucocorticoids stimulated both the differentiated phenotype of chondrocytes and the proliferation of rabbit costal chondrocytes in culture. Moreover, the effect of glucocorticoids was primarily on the differentiated phenotype of chondrocytes and its effect on proliferation was permissive.
通过[35S]硫酸盐和[3H]葡萄糖胺的掺入量判断，氢化可的松刺激了汇合静止培养的兔肋软骨细胞中糖胺聚糖（GAG）的合成，这是软骨表型的一个特征。氢化可的松还刺激了[3H]丝氨酸掺入蛋白聚糖。氢化可的松对GAG合成的刺激呈剂量依赖性，在生理浓度10（-7）M时达到最大。氢化可的松还刺激了对数生长期培养物中的GAG合成。在这种情况下，在10（-6）M的浓度下观察到其最大效应。汇合培养中氢化可的松刺激的GAG合成增加幅度大于对数期培养。氢化可的松剂量依赖性地刺激DNA合成，其效应在生理浓度下即可观察到。然而，与GAG合成相反，在无血清培养基中未观察到氢化可的松对DNA合成的刺激。氢化可的松还增加了蛋白质合成和细胞数量。地塞米松也刺激了GAG和DNA的合成。这些结果表明，糖皮质激素刺激了培养的软骨细胞的分化表型和兔肋软骨细胞的增殖。此外，糖皮质激素的作用主要在于软骨细胞的分化表型，其对增殖的作用是允许性的。
