Takigawa M, Takano T, Nakagawa K, Sakuda M, Suzuki F
Department of Biochemistry and Calcified-Tissue Metabolism, Osaka University, Japan.
Arch Oral Biol. 1988;33(12):893-9. doi: 10.1016/0003-9969(88)90019-2.
Hydrocortisone stimulated glycosaminoglycan (GAG) synthesis, a cartilage phenotype, in chondrocytes from mandibular condylar cartilage (MCC), nasal septal cartilage (NSC) and sphenooccipital synchondrosis (SOS). These stimulations were dose- and time-dependent, being maximal 27 h after addition of 10(-7) M hydrocortisone. The maximal induced increase of GAG synthesis was about 100%, 50% and 20% that of non-stimulated MCC, SOS and NSC chondrocytes, respectively. When stained with toluidine blue, all three types of cortisone-treated chondrocytes showed stronger metachromasia than non-treated controls. DNA synthesis was also increased by hydrocortisone, reaching a maximum 20 h after the addition; stimulation was also dose-dependent and maximal at a concentration of 10(-6) M. The maximal increase in DNA synthesis was 200% in NSC chondrocytes, 90% in SOS chondrocytes, and slight in MCC chondrocytes. However, there was no stimulation of DNA synthesis in serum-free medium, in contrast to that of GAG synthesis. These observations suggest that hydrocortisone regulates craniofacial growth by controlling the differentiation of these chondrocytes directly and their proliferation indirectly, and that the difference in their responses to hydrocortisone may reflect different responses in vivo.
氢化可的松刺激了来自下颌髁突软骨（MCC）、鼻中隔软骨（NSC）和蝶枕软骨结合（SOS）的软骨细胞中糖胺聚糖（GAG）的合成，这是一种软骨表型。这些刺激呈剂量和时间依赖性，在添加10⁻⁷ M氢化可的松后27小时达到最大值。GAG合成的最大诱导增加分别约为未刺激的MCC、SOS和NSC软骨细胞的100%、50%和20%。用甲苯胺蓝染色时，所有三种经可的松处理的软骨细胞均显示出比未处理的对照更强的异染性。氢化可的松也增加了DNA合成，在添加后20小时达到最大值；刺激也呈剂量依赖性，在浓度为10⁻⁶ M时最大。NSC软骨细胞中DNA合成的最大增加为200%，SOS软骨细胞中为90%，MCC软骨细胞中则轻微增加。然而，与GAG合成不同，在无血清培养基中未观察到DNA合成的刺激。这些观察结果表明，氢化可的松通过直接控制这些软骨细胞的分化和间接控制其增殖来调节颅面生长，并且它们对氢化可的松反应的差异可能反映了体内的不同反应。
