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酵母羧肽酶Y突变体的研究及第二种羧肽酶活性的证据。

Studies on a carboxypeptidase Y mutant of yeast and evidence for a second carboxypeptidase Activity.

作者信息

Wolf D H, Weiser U

出版信息

Eur J Biochem. 1977 Mar 1;73(2):553-6. doi: 10.1111/j.1432-1033.1977.tb11350.x.

DOI:10.1111/j.1432-1033.1977.tb11350.x
PMID:403073
Abstract

Immunological studies on the carboxypeptidase Y mutant prcl-l of Saccharomyces cerevisiae revealed the origin of mutation in the structural gene of carboxypeptidase Y. The absence of carboxypeptidase Y has no effect on growth, even after drastic changes of growth conditions. A double mutant (prc 1- leu2-) lacking carboxypeptidase Y and auxotrophic for leucine is able to grow on the peptide benzyloxycarbonylglycylleucine (Cbz-Gly-Leu) as sole nitrogen source, indicating the existence of a second carboxypeptidase. Using a new peptidase test, the existence of this second enzyme, called carboxypeptidase S, was confirmed biochemically.

摘要

对酿酒酵母羧肽酶Y突变体prc1-1的免疫学研究揭示了羧肽酶Y结构基因中的突变起源。即使在生长条件发生剧烈变化后，羧肽酶Y的缺失对生长也没有影响。缺乏羧肽酶Y且对亮氨酸营养缺陷的双突变体（prc1-leu2-）能够以肽苄氧羰基甘氨酰亮氨酸（Cbz-Gly-Leu）作为唯一氮源生长，这表明存在第二种羧肽酶。通过一种新的肽酶测试，从生化角度证实了这种名为羧肽酶S的第二种酶的存在。

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