Zubenko G S, Park F J, Jones E W
Genetics. 1982 Dec;102(4):679-90. doi: 10.1093/genetics/102.4.679.
Yeast cells that inherit mutations at the PEP4 locus exhibit a pronounced phenotypic lag in the expression of the mutant phenotype imparted by these mutations. This lag appears to extend to all of the enzymes that are affected by the pep4-3 mutation. For at least two of the enzymatic activities, phenotypic lag shows mitotic cosegregation. Phenotypic lag is found for meiotic progeny and for mitotic segregants from heterokaryons. The phenotypic lag in the expression of the carboxypeptidase Y deficiency is abolished by nonsense mutations in either PRC1, the structural gene for carboxypeptidase Y, or PRB1, the structural gene for proteinase B. Models to explain these observations are proposed.
在PEP4位点发生突变的酵母细胞，在表达由这些突变赋予的突变表型时表现出明显的表型延迟。这种延迟似乎延伸到所有受pep4 - 3突变影响的酶。对于至少两种酶活性，表型延迟显示有丝分裂共分离。在减数分裂后代和异核体的有丝分裂分离子中都发现了表型延迟。羧肽酶Y缺陷表达中的表型延迟可被羧肽酶Y的结构基因PRC1或蛋白酶B的结构基因PRB1中的无义突变消除。本文提出了解释这些观察结果的模型。
